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1、JournalofBiotechnology145(2010)160–167ContentslistsavailableatScienceDirectJournalofBiotechnologyjournalhomepage:www.elsevier.com/locate/jbiotecTruncatedderivativesofamultidomainthermophilicglycosylhydrolasefamily10xylanasefromThermotogamaritimarevealstructurerelatedactiv
2、ityprofilesandsubstratehydrolysispatternsPriscillaVerjansa,EmmieDorneza,MartienSegersa,StevenVanCampenhouta,KristelBernaertsb,TimBeliëna,JanA.Delcoura,ChristopheM.Courtina,∗aDepartmentofMicrobialandMolecularSystems,KatholiekeUniversiteitLeuven,KasteelparkArenberg20,B-3001,
3、Leuven,BelgiumbDepartmentofChemicalEngineering,KatholiekeUniversiteitLeuven,W.deCroylaan46,B-3001Leuven,BelgiumarticleinfoabstractArticlehistory:EfficientheteroxylandegradationinthecontextofeconomicallyfeasiblelignocellulosicbiomassReceived30June2009biorefiningrequiresxylan
4、olyticenzymeswithoptimalthermostabilityandspecificity.Therefore,Receivedinrevisedform16October2009thestructureactivityrelationshipofamodularthermophilicglycosidehydrolasefamily10xylanaseAccepted21October2009(xylanaseAfromThermotogamaritimaMSB8,rXTMA)wasinvestigatedthroughc
5、onstructionofsixtruncatedderivatives,lackingatleastoneofthe2N-and/or2C-terminalmodules.ThetemperaturesforKeywords:optimalactivityandstabilityofthexylanaseswerestronglyinfluencedbythepresenceofthedifferentXylanase◦◦modulesandrangedfrom60to80Cand50to80C,respectively.Incontra
6、st,thepHforoptimalThermophilicityactivitywasonlyslightlyaffected(pH6.0to7.0).Thetestedxylanasesretainedover80%activityafter2hThermostabilitypre-incubationat50◦CbetweenpH5.0and11.0.Mostunexpectedly,changesinthemodularstructureSubstratehydrolysisInhibitionsensitivityledtoa2
7、6-foldwiderangeofspecificactivitiesoftheenzymestowardsxylohexaose,whiletheactivityBiomasstowardsinsolublepolymericheteroxylanwascomparableforallbutonexylanase.rXTMAC,lackingBiorefiningtheC-terminalmodules,hada60%higherspecificactivitytowardsthelattersubstratethanthewildtype
8、enzyme.TheseresultsshowthatkeypropertiesofXTMAcanbetunedtoallowforoptimalperformanceoftheenzymei
