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1、GModelMOLCAB-2345;No.ofPages7ARTICLEINPRESSJournalofMolecularCatalysisB:Enzymaticxxx(2011)xxx–xxxContentslistsavailableatSciVerseScienceDirectJournalofMolecularCatalysisB:Enzymaticjournalhomepage:www.elsevier.com/locate/molcatbPurificationandsomepropert
2、iesoflow-molecular-weightextremehalophilicxylanasefromChromohalobactersp.TPSV101B.aaba,∗Prakash,M.Vidyasagar,S.K.Jayalakshmi,K.SreeramuluaDepartmentofBiochemistry,GulbargaUniversity,Gulbarga585106,Karnataka,IndiabAgriculturalResearchStation,Universityo
3、fAgriculturalSciences-Raichur,Gulbarga585106,IndiaarticleinfoabstractArticlehistory:AnextremehalophilicxylanasewaspurifiedfromculturesofChromohalobactersp.TPSV101byultrafil-Received18February2011tration,hydroxylapatiteandgelfiltrationchromatography.SDS-PA
4、GEofthexylanaseshowedanapparentReceivedinrevisedformhomogeneity◦Septemberandmolecularweightof15kDa.ThexylanasehadmaximumactivityatpH9.0and65C201124inthepresenceof15–25%NaClandwasstableintherangeofpH7.0–9.0,temperaturebetween50andAccepted5October201170◦
5、C.◦AvailableonlinexxxTheenzymewasstableat50–65Cfor1hretaining100%activityandbyretaining60%activityat◦2+2+2+80C.ThexylanasewascompletelyinhibitedbyHgionsandwaspartiallyinhibitedbyCa,Cuand2+2+2+2+Keywords:Pbions,whereasZn,MnandCoionsenhanceditsactivity.B
6、othEGTAandEDTAenhanceditssp.activity.Itwasactiveinsolutionscontainingwater-insolubleorganicsolventsandosmolytes.KineticTPSV101ChromohalobacterexperimentsindicatedthattheenzymehadKXylanasemandVmaxvaluesof0.2mg/mland1.17mol/ml/minforoatOsmolytesspeltxyl
7、an.Themajorproductsoftheoatspeltxylanhydrolysiswerexyloseandxylobiose;afterprolongedincubationxylosewasthemajorendproduct.OatspeltxylanXylobiose©2011ElsevierB.V.Allrightsreserved.1.IntroductionWhiletherehavebeennumerousreportsonextracellularxylanasesfr
8、omnon-halophilicbacteria,verylimitedinforma-Hemicellulosesarelow-molecular-weightpolysaccharidestionisavailableonxylanasesfromhalophilicspecies.Xylanolyticfoundincloseassociationwithcelluloseandligninintheplantactivitieshavebeenreported
