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1、InnovativeFoodScienceandEmergingTechnologies12(2011)187–196ContentslistsavailableatScienceDirectInnovativeFoodScienceandEmergingTechnologiesjournalhomepage:www.elsevier.com/locate/ifsetXylanaseBfromthehyperthermophileThermotogamaritimaasanindicatorfortemperaturegra
2、dientsinhighpressurehightemperatureprocessingaaabbLiesbethVervoort,IeselVanderPlancken,TaraGrauwet,PriscillaVerjans,ChristopheM.Courtin,aa,⁎MarcE.Hendrickx,AnnVanLoeyaLaboratoryofFoodTechnology,LeuvenFoodScienceandNutritionResearchCenter(LFoRCe),DepartmentofMicrobi
3、alandMolecularSystems(M²S),KatholiekeUniversiteitLeuven,KasteelparkArenberg22box2457,B-3001Heverlee,BelgiumbLaboratoryofFoodChemistryandBiochemistry,LeuvenFoodScienceandNutritionResearchCenter(LFoRCe),DepartmentofMicrobialandMolecularSystems(M²S),KatholiekeUniversi
4、teitLeuven,KasteelparkArenberg22box2463,B-3001Heverlee,BelgiumarticleinfoabstractArticlehistory:Withinthescopeofhighpressurefoodsterilization,animportantissuethatshouldbetakenintoaccountinReceived17September2010refiningprocessandequipmentdesignisthetime-andposition-
5、dependenttemperaturegradientthatexistsAccepted12January2011throughoutthepressurevesselandtheproductload.Sinceenzymesfromthermophilicmicroorganismsshowgoodprospectsforthedevelopmentofindicatorstomapoutthetemperaturenon-uniformityinhighEditorProofReceiveDate10Februar
6、y2011pressurehightemperature(HPHT)processing,inthiswork,thepotentialofxylanaseBfromThermotogamaritima(XTMB)wasinvestigated.Itsinactivationatisothermal–isobaricconditionswasbestdescribedbyaKeywords:Pressure–temperature–timeindicator(pTTI)first-ordermodel.Thepressured
7、ependenceoftheDvalueswasnegligibleatHPHT,thetemperatureHighpressurehightemperature(HPHT)dependencehoweverwassubstantial.TheThermalDeathTime(TDT)model,anditscorrespondingprocessingparameters,describingthislargetemperaturedependenceweresuccessfullyvalidatedunderdynam
8、icHighpressuresterilizationprocessingconditions,relevantforindustrialHPHTapplications.TemperatureuniformityIndustrialrelevance:Despiteextensivere