tracking the prokaryotic diversity in acid mine drainage-contaminated

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1、MineralsEngineering24(2011)709–718ContentslistsavailableatScienceDirectMineralsEngineeringjournalhomepage:www.elsevier.com/locate/minengTrackingtheprokaryoticdiversityinacidminedrainage-contaminatedenvironments:AreviewofmolecularmethodsBidyutR.Mohapatr

2、a⇑,W.DouglasGould,OrlandoDinardo,DavidW.KorenCANMETMiningandMineralSciencesLaboratory,NaturalResourcesCanada,555BoothStreet,Ottawa,Ontario,CanadaK1A0G1articleinfoabstractArticlehistory:Thenegativeimpactofacidminedrainage(AMD)onpublicandenvironmentalhea

3、lthbydegradingtheReceived11January2011aquaticandterrestrialecosystemwithadrasticdecreaseinpHandelevatedlevelsoftoxicheavymetals,Accepted17March2011metalloidsandradionuclidesnecessitatethedevelopmentofenvironmentallysustainabletechnologiesAvailableonlin

4、e9April2011toremediateAMD.Thedevelopmentofappropriatestrategiesforcontrollingand/orabatingthedetri-mentaleffectsofAMDinnaturalandminingenvironmentsprimarilydependsonthediversityandcom-Keywords:positionsofthelocalacidophilicmicrobialcommunities(thosewhi

5、chcangrowatpH63)whichcatalyzeAcidminedrainagethereactionsofAMDbyproductionofsulfuricacidandferriciron.Robustmethod(s)totracktheAMD-BioleachingpromotingmicrobialcommunitieswillnotonlyprovideinformationabouttheirecophysiologicalroleinHeavymetalsMolecular

6、methodsextremeenvironmentsbutalsohelpsustainthereliabilityofremediationtechnologies.Thispaperpro-ProkaryotesvidesanoverviewonthephylogeneticdiversityofprokaryotespresentinAMD-impactedenvironments,Radionuclidesanddifferentmolecularmethodsthathavebeenuse

7、dtotrackthediversityoftheseacidophiles.Addi-tionally,thehigh-throughputmethods(metagenomics,metaproteomicsandmicroarrays)thatlinkpro-karyoticphylogenytotheirfunctioninAMDsystemsarealsodiscussed.Ó2011ElsevierLtd.Allrightsreserved.Contents1.Introduction.

8、........................................................................................................7102.PhylogeneticdiversityofprokaryoticcommunitiesinAMD-impactedenvironments...............................................7103.Mole