development and characterization of microsatellite markers

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1、ConservationGenetResour(2009)1:237239DOI10.1007/s12686-009-9058-zTECHNICALNOTEDevelopmentandcharacterizationofmicrosatellitemarkersforcastor(RicinuscommunisL.),animportantoleaginousspeciesforbiodieselproductionMiklosMaximilianoBajayÆJose´BaldinPinheiroÆCarlosEduardoArau´joBatista

2、ÆMa´rciaBarretoMedeirosNobregaÆMariaImaculadaZucchiReceived:24June2009/Accepted:30June2009/Publishedonline:25July2009ÓSpringerScience+BusinessMediaB.V.2009AbstractCastor(RicinuscommunisL.)isanimportantCastor(RicinuscommunisL.,Euphorbiaceae,2n=20),oleaginousplantfrombotheconomican

3、dsocialpointsofwhoseorigincenterisprobablyEthiopia,iscultivatedinview.Theseedscontainanoilwithexcellentpropertiesformanytropicalandsubtropicalregionsoftheworldindustrialuses.Thispaperpresentsthemainresultsofa(Govaertsetal.2000).Theseeds,leavesandstemofcastorstudyaimingtodevelopmi

4、crosatellitemarkersforcastor.arepoisonouswhenconsumedbyhumansandlivestock,Twelvenewpolymorphicmicrosatellitemarkerswerebecauseofthepresenceofricin,themajortoxicalkaloidisolatedandcharacterizedin38genotypesaccessionsfrompresentintheplant(Zimmermanetal.1958).Intheotherthecastorgerm

5、plasmoftheBrazilianAgriculturalhand,theseedshaveaveryhighconcentration(more45%)ResearchCompany(EMBRAPA).Knowledgeontheofexcellentoil,idealfortheproductionofbiodiesel(JeonggeneticdiversityofcastorcanbeusedtogainabetterandPark2009).Istheonlyvegetableoilsolubleinalcohol,understandin

6、gongeneticdiversityconservation,andpresentinghighviscosityandrequiringlessheatingthangermplasmmanagement,guidingbreedingprogramsandothersoilsduringtheproductionofbiodiesel(Beltraoconservationstrategies.2003).Castoroilhasmanyotherusesrangingfromcos-meticsand,medicinestolubricantsu

7、tilizedinaircraftandKeywordsGermplasm
Geneticdiversity
spacerockets.SSRsandconservationLossofgeneticdiversityisaprobleminthecaseofsomeimportantspeciesforagriculture,sinceancientcul-tivars(orlandraces)andwildrelativesofdomesticatedspeciesarebeinglostasmodernvarietiesbecomeadoptedb

8、yfarmers.Thishasledtocallsforgeneticcons