identification+and+characterization+of+43+microsatellite+markers+derived+from+expressed+sequence+tags+of+the+sea+cucumber+（apostichopus+japonicus）

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1、J．0ceanUniv．China(OceanicandCoastalSeaResearch)D0I10．1007／s11802—011一l814—5ISSN1672—5182，201110(2)：185—190tp．'／／www．oldc．edu．cn／xb)wb／E-mail：xbywb@ouc．edu．cnIldentifiticationandCharact,e~rizationofl43MilcrosatelfliteMarkersDerivedfromExpressedSequenceTagsoftheSeaCucumber(Apostichopusjaponicus)J

2、IANGQun，LIQi，YUHong，andKONGLingfengFisheriesCollege，OceanUniversityofChina，Qingdao266003，China(ReceivedDecember6，2010：revisedMarch14，2011acceptedMarch18，2011)⑥OceanUniversityofChina．SciencePressandSpring—VerlagBerlinHeidelberg2011AbstractTheseacucumberApostichopus／aponicusisacommerciallyandecol

3、ogicallyimportantspeciesinChina．Atotalof3056potentialunigenesweregeneratedafterassembling7597A．japonicusexpressedsequencetags(ESTs)downloadedfromGen—Bank．Twohundredandfiftymicrosatellite—containingESTsf8．18％1and299simplesequencerepeats(ssRs1weredetected．TheaveragedensityofSSRswaslper7．403kb0fES

4、Tafterredundancyelimination．Di—nucleotiderepeatmotifsappearedtobethemostabundanttypewithapercentageof69．90％．0fthe126primerpairsdesigned．90amplifiedtheexpectedproductsand43showedpolymorphismin30individualstested．Thenumberofallelesper1OCUSrangedfrom2to26withanaverageof7．0alleles．andtheobservedand

5、expectedheterozygositiesvariedfrom0．067to1．000andfrom0．066to0．959．respectively．ThesenewEST—derivedmicrosatellitemarkerswouldprovidesufficientpolymorphismforpopulationgeneticstudiesandgenomemappingofthisseacucumberspecies．Keywordsseacucumber；Apostichopusjaponicus；microsatellite；ESTcomparativemap

6、pingamongdistantevolutionaryorgan。1Introductionisms(Serapioneta1．，2004)．Expressedsequencetags(ESTs)aresingle-passsequencesofrandomlypickedTheseacucumber(Apostichopusjaponicus)，acom—complementaryDNAclones(Adamseta1．．19911．ThesemonbenthicdetritusfeederofthefamilyStichopodidae，sequencesrepresenttr

7、anscribedsequencesofthegenomedistributesalongthecoastsofChina，Japan，Korea，andorgenesandsomeofthemareconnectedwithknownRussianFarEast．Duringthepastdecade．farmingof．genes．Asaresult，ESTdatabasesarearichsourcefor7aponicushasbecomewide