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1、GModelEMT-8281;No.ofPages9ARTICLEINPRESSEnzymeandMicrobialTechnologyxxx(2011)xxx–xxxContentslistsavailableatScienceDirectEnzymeandMicrobialTechnologyjournalhomepage:www.elsevier.com/locate/emtCharacterizationandoptimizationof-galactosidaseimmobilizationprocessonamixed-matrixmembr
2、anePetera,baaaa,ba,∗Jochems,YaminiSatyawali,SandraVanRoy,WimDoyen,LudoDiels,WinnieDejongheaSeparationandConversionTechnology,FlemishInstituteforTechnologicalResearch(VITO),Boeretang200,2400Mol,BelgiumbUniversityofAntwerp,DepartmentofBioscienceEngineering,Groenenborgerlaan171,2020A
3、ntwerp,BelgiumarticleinfoabstractArticlehistory:-Galactosidaseisanimportantenzymecatalyzingnotonlythehydrolysisoflactosetothemonosaccha-Received31January2011ridesglucoseandgalactosebutalsothetransgalactosylationreactiontoproducegalacto-oligosaccharidesReceivedinrevisedform1June20
4、11(GOS).Inthisstudy,-galactosidasewasimmobilizedbyadsorptiononamixed-matrixmembranecon-6June2011Acceptedtaining2zirconiumdioxide.Themaximum-galactosidaseadsorbedonthesemembraneswas1.6g/m,however,2Keywords:maximalactivitywasachievedatanenzymeconcentrationofaround0.5g/m.Thetestsco
5、n-ducted-Galactosidasetoinvestigatetheoptimalimmobilizationparameterssuggestedthathigherimmobilizationcanbeachievedunderextremeparameters(pHandtemperature)buttheactivitywasnotretainedatsuchMixed-matrixmembraneextremeEnzymeoperationalparameters.Theinvestigationsonimmobilizedenzyme
6、sindicatedthatnorealshiftImmobilizationoccurredinitsoptimaltemperatureafterimmobilizationthoughtheactivityincaseofimmobilized◦enzymewasbetterretainedatlowertemperature(5C).Ashiftof0.5unitwasobservedinoptimalpHSpecificactivityafterimmobilization(pH6.5to7).Perhapsthemoststrikingresul
7、tsarethekineticparametersoftheBiocatalyticmembraneimmobilizedenzyme;whiletheMichaelisconstant(Km)valueincreasedalmosteighttimescomparedtothefreeenzyme,themaximumenzymevelocity(Vmax)remainedalmostconstant.©2011ElsevierInc.Allrightsreserved.1.IntroductionIncaseof-galactosidase,enzy
8、meparametersandpriceeven-tuallyde
