DB21T 2734.1-2017 病菌PCR 分型诊断方法 第1部分：大肠杆菌PCR 检测方法

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1、ICS11.220B41DB21辽宁省地方标准DB21/T2734.1—2017病菌PCR分型诊断方法第1部分：大肠杆菌PCR检测方法MethodofPCRtypingdiagnosisforbacterialPart1：MethodofPCRdetectionforEscherichiacoli2017-02-23发布2017-03-23实施辽宁省质量技术监督局发布DB21/T2734.1—2017目次前言................................................................................II1范围.

2、.............................................................................12规范性引用文件....................................................................13缩略语............................................................................14原理.....................................................

3、.........................15仪器和器材........................................................................16试剂和材料........................................................................17操作步骤..........................................................................27.1样品的采集、前处理、存放与运输..........

4、......................................27.1.1采样注意事项..............................................................27.1.2采样工具..................................................................27.1.3内脏样品的采集与前处理....................................................27.1.4内容物的采集与前处理...................

5、...................................27.1.5粪便的采集与前处理........................................................27.1.6血液......................................................................27.1.7大肠杆菌纯培养物..........................................................37.1.8保存与运输.........................

6、.......................................37.2PCR检测.......................................................................37.2.1DNA提取...................................................................37.2.2PCR.......................................................................37.2.2.2PCR反应条件

7、...........................................................37.2.2.3电泳..................................................................48试验成立的条件....................................................................49结果判定..............................