抗酸染色电子版本.doc

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1、精品好文档，推荐学习交流抗酸染色一般步骤1）初染用玻片夹夹持涂片标本，滴加石炭酸复红2-3滴，在火焰高处徐徐加热，切勿沸腾，出现蒸汽即暂时离开，若染液蒸发减少，应再加染液，以免干涸，加热3-5分钟，待标本冷却后用水冲洗。2）脱色3%盐酸酒精脱色30秒~1分钟；用水冲洗。3）复染用碱性美兰溶液复染1分钟，水洗，用吸水纸吸干后用油镜观察。4抗酸染色的染液配制1、石炭酸复红碱性复红酒精饱和溶液10mL5%石炭酸90mL2、3%盐酸酒精浓盐酸3mL95%酒精97mL3、吕氏美兰液美兰酒精饱和液30mL氢氧化钾（1:10000）100mL 抗酸染色的原理分枝杆菌的细胞壁内含有大量的脂质，

2、包围在肽聚糖的外面，所以分枝杆菌一般不易着色，要经过加热和延长染色时间来促使其着色。但分枝杆菌中的分枝菌酸与染料结合后，就很难被酸性脱色剂脱色，故名抗酸染色。齐-尼氏抗酸染色法是在加热条件下使分枝菌酸与石炭酸复红牢固结合成复合物，用盐酸酒精处理也不脱色。当再加碱性美兰复染后，分枝杆菌仍然为红色，而其他细菌及背景中的物质为蓝色。初一英语（下）动词的一般过去时专练一、写出下列动词的过去式仅供学习与交流，如有侵权请联系网站删除谢谢2精品好文档，推荐学习交流1.is_____2.are_____3.have______4.go_____5.do_______6.stop7.see___

3、_8.sit_____9.stay___10.eat________11.thank_______12.decide_______13.feel_______14.make_________15.cry________二、用括号中所给词的适当形式填空。1.I___________(have)anexcitingpartylastweekend.2.--_____________she_____________(play)herguitar（吉他）yesterday?--No,she_____________.3.—What___________Tom__________(do)

4、onSaturdayevening?--He_________(watch)TVand__________(read)aninterestingbook.4.Theyall____________(go)tothemountainsyesterdaymorning.5.She_____________(notvisit)herauntlastweekend.She____________(stay)athomeand___________(do)somecleaning.6.–When__________you____________(write)thissong?--I___

5、_________(write)itlastyear.7.Myfriend,Carol,____________(study)forthemathstestand____________(practise)Englishlastnight.8.--__________MrLi_____________(do)theprojectonMondaymorning.--Yes,he_____________.9.–How_______________(be)Jim’sweekend?--It_________________(benot)bad.10.--__________(be)

6、yourmotherasalesassistant（售货员）lastyear?--No,she______________.11.I____________(go)totheparklastweekend.12.When___________Sam____________(do)hishomeworklastnight?13.Linda_____________(clean)theroomyesterday.14.Mygrandfather_____________(be)sicklastweek.15.Heoften______(play)basketballaftersch

7、oolwhenhewasastudent.16.They____________(be)veryhappytohearthegoodnewsyesterday.17.I_______________(see)himtoday.Hewenttoseehisgrandmother.18.I______________(be)busylastweek.19.Mary____________(notvisit)herauntlastmonth.20.There____________(be)alot