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1、当归抗氧化活性与其酚性相关性分析广州中医药大学李熙灿摘要当归（AngelicaSinensis）是一种很常用的中药。本研究采用溶剂提取法得到六种当归提取物，然后，测定了这六种提取物的抗氧化活性，包括：DPPH(1,1-Diphenyl-2-picrylhydrazylradical)清除法,ABTS(2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonicaciddiammoniumsalt))清除法,还原能力法,•O2–自由基清除法，和抗脂质过氧化法。再采用相应的方法测定六种提取物的多酚含量，阿魏酸含量，和咖啡酸含量
2、。据此，将六提取物的抗氧化活性水平（1/IC50）与其化学含量做相关图，得到各种相关系数（R）。结果表明：R=0.642～0.941，平均值为0.839。这说明，当归的抗氧化活性与其内含的多酚，阿魏酸和咖啡酸含量正相关。换句话说，当归的抗氧化剂来自于酚类化合物，尤其是阿魏酸和咖啡酸。[文献来源]XicanLi,XiaotingWu,LingHuang.CorrelationBetweenAntioxidantActivitiesandPhenolicContentsofRadixAngelicaeSinensis(Danggui),Molecules,200
3、9,14:5349-5361.AbstractBackgroundAsatypicalChineseherbalmedicine,rhizomaCimicifugae(RC,升麻inChinese)possessesvariouspharmacologicaleffectsinvolvedinantioxidantactivity.However,itsantioxidantactivityhasnotbeenreportedsofar.Theaimofthepresentstudywastosystematicallyevaluatetheantioxid
4、antabilityofRCinvitro,thendiscussthemechanism.MethodsFirstly,fiveRCextracts(i.e.petroleumetherextractPERC,ethylacetateextractEARC,absoluteethanolextractAERC,95%ethanolextract95ERC,andwaterextractWRC)werepreparedanddeterminedbyvariousantioxidantmethods,includinganti-lipidperoxidatio
5、n,protectionagainstDNAdamage,•OHscavenging,•O2-scavenging,DPPH•(1,1-diphenyl-2-picryl-hydrazlradical)scavenging,ABTS+•(2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonicacidradicalion)scavenging,Cu2+-chelating,andFe3+reducingassays.Subsequently,wemeasuredthechemicalcontentsoffiveRCe
6、xtracts,includingtotalphenolics,totalsaponins,totalsugars,caffeicacid,ferulicacidandisoferulicacid.Finally,wequantitativelyanalyzedthecorrelationsbetweenantioxidantlevels(1/IC50values)andchemicalcontents.ResultsInallantioxidantassays,fiveRCextractsincreasedtheantioxidantlevelsinado
7、se-dependentmanner.However,theirantioxidantlevels(IC50values)andchemicalcontentssignificantlydifferedfromeachother.Quantitativeanalysisofthecorrelationshowedthattotalphenolicwasofsignificantpositivecorrelations(averageRvaluewas0.56)withantioxidantlevels;Incontrast,totalsugarsandtot
8、alsaponinshadnopositivecor
