MBL调节白假丝酵母菌相态转化的作用及机制的初步分析-论文.pdf

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1、翟晶晶等MBL调节白假丝酵母菌相态转化的作用及机制的初步分析第9期·1153·doi：10．3969／j．issn．1000-484X．2014．09．001·基础免疫学·MBL调节白假丝酵母菌相态转化的作用及机制的初步分析①翟晶晶王明永②凌明智王凡平郭康③宋士军③段巨洪张娜李梦杰左萌洁赵雯瑕(新乡医学院医学检验学院，新乡453003)中图分类号R392．1文献标志码A文章编号1000-484X(2014)09·1153-05[摘要]目的：探讨甘露聚糖结合凝集素(Mannan—bindinglectin，MBL)对白假丝酵母菌(Candidaalbicans，C．albicans)相态转

2、化的调节作用及机制。方法：以人MBL处理ealbicans2、4、8h，37oC200r／min震荡培养，倒置相差显微镜下观察C．albicans的菌丝形成情况；FACS分析MBL与Dalbicans的结合情况；RT—PCR分析calbicans相态转化调控基因HWP1、DDR48的表达。结果：倒置相差显微镜观察发现MBL在早期(2～4h)能够抑制ealbicans酵母相(Yeastform，Y)向菌丝相(Hyphalform，H)转化；FACS分析表明MBL能够以ca浓度依赖关系与C．albicans细胞结合；RT．PCR结果表明MBL能够减少C．albicans相态转化调控基因HWP

3、1的表达，而其对DDR48表达的影响尚无规律可循。结论：MBL能够通过调控HWP1的表达抑制C．albicans酵母相向菌丝相转化。[关键词]甘露聚糖结合凝集素；白假丝酵母菌；相态转化；基因表达PreliminaryanalysisofefectsandmechanismsonMBLregulatingdimorphicswitchingofCandidaalbicansZHAIJing一如，WANG一，LINGMing一，WANGFan—P，GUOKang，SONGShi—Jun，DUANJu—Hong，ZHANGNa，LIMeng—Jie，ZUOMeng—Jie，ZHAO耽n—Xia

4、．SchoolofLaboratoryMedicine，XinxiangMedicalUniversity，Xinxiang453003，China[Abstract]Objective：Toinvestigatetheeffectsandmechanismsofmannan—bindinglectin(MBL)regulateCandidaalbicans(C．albicans)dimorphicswitching．Methods：C．albicanswereprocessed2h，4h，8hbyhumanMBL，andwereshakedandculturedin37℃200r／mi

5、naswellascontrolgroups．thenweobservedthehyphaeformationofCalbicansundertheinvertedphasecontrastmicroscope．ThebindingofMBLandC．albicanswereanalyzedbyFACS．ThelevelsofealbicansdimorphicswitchingregulatorygenesHWP1，DDR48onealbicansweredeterminedbyRT—PCR．Results：InvertedphasecontrastmicroscopeSobserva

6、tionindicatedthatMBLinhibitsC．albicansYeastformtransformtoHyphalformintheearly(2h，4h)．FACSshowedthatCa2+-dependentbindingunderlyinginteractionofMBLwithCalbicanscells．RT-PCRshowedthatMBLdown—regulatetheexpressionofC．albicansphasetransitionregulatorygenesHWP1，andyettherewasnorulestofollowitsefecton

7、DDR48expression．Conclusion：MBLinhibitsealbicansYeastformtransformtoHyphalformthroughregulatingtheexpressionofHWP1．[Keywords]Mannan-bindinglectin；Candidaalbicans；Dimorphicswitching；Geneexpression甘露聚糖结合凝集素(Mannan-binding