1、pET-26b(+)编号载体名称北京华越洋生物VECT4140pET‐26b(+)pET26b载体基本信息别名:pET26b, pet‐26b(+), pET 26b(+)质粒类型:大肠杆菌蛋白表达表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:5360bp5' 测序引物:T75' 测序引物序列:5'‐TAATACGACTCACTATAGGG‐3'载体标签:C‐His载体抗性:卡那(Kanamycin)备注:含有pelB信号肽，蛋白表达时，能够将目的基因定位于细胞周质。稳定性:瞬时表达Transient组成型:组成型Constitutive病毒/非
2、病毒:非病毒pET26b载体质粒图谱和多克隆位点信息pET26b载体简介The pET‐26b(+) vector (Cat. No. 69862‐3) carries an N‐terminal pelB signal sequence for potential periplasmiclocalization, plus optional C‐terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered b
3、y the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing s
4、ingle‐stranded DNA that corresponds to the coding strand. Therefore, single‐stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337‐3).pET26b载体序列ORIGIN1ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAA61GGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAG
