1、pET-21a(+)编号载体名称北京华越洋生物VECT4170pET‐21a(+)pET21a载体基本信息别名:pET21a, pet 21a质粒类型:大肠杆菌蛋白表达表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:5443bp5' 测序引物:T75' 测序引物序列:5'‐TAATACGACTCACTATAGGG‐3'载体标签:N‐T7, C‐His载体抗性:Ampicillin备注:Same as pET24abcd(+) but ampR; a,b,c,d vary by MCS; The f1 or
2、igin is oriented so that infection with helper phage will produce virions containing single‐stranded DNA that corresponds to the coding strand.稳定性:瞬时表达Transient组成型:组成型Constitutive病毒/非病毒:非病毒pET21a载体质粒图谱和多克隆位点信息pET21a载体简介The pET‐21a‐d(+) vectors carry an N‐term
3、inal T7•Tag® sequence plus an optional C‐terminal His•Tag® sequence. These vectors differ from pET‐24a‐d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by
4、the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce
5、virions containing single‐stranded DNA that corresponds to the coding strand. Therefore, single‐stranded sequencing should be performed using the T7 terminator primer.pET21a载体序列ORIGIN1ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAA61GGGGTTATGCTAG
