2、O Expression System produces the highest levels of soluble protein in E. coli. It utilizes a small ubiquitin‐related modifier (SUMO) fusion, belonging to the growing family of ubiquitin‐related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is invo
3、lved in the stabilization and localization of proteins in vivo. After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP‐1) protease at the carboxyl terminal, producinga native protein*. The Champion pET SUMO Protein and Peptide Expression System offers:
4、 Greatly enhanced solubility with an N‐terminal SUMO fusionHighly efficient cleavage‐produces native protein of interest with SUMO (ULP‐1) protease*Highly specific cleavage‐eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificant
5、ly increased stability with SUMO fusion‐can be used for small peptide productionT7lac promoter for high‐level protein expressionN‐terminal 6xHis tag for protein detection and purification.pETsumo载体序列CAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTC
