1、pET-14b编号载体名称北京华越洋生物VECT4150pET‐14bpET14b载体基本信息别名:pET14b, pet 14b质粒类型:大肠杆菌蛋白表达表达水平:高克隆方法:多克隆位点，限制性内切酶载体大小:4671bp5' 测序引物:T75' 测序引物序列:5'‐TAATACGACTCACTATAGGG‐3'载体标签:N‐His, N‐thrombin载体抗性:Ampicillin备注:Hosts: E.coli. Related vectors: pBR322.稳定性:瞬时表达Transient组成型:组成型Constitutive病毒/非病毒:非病毒pET14
2、b载体质粒图谱和多克隆位点信息pET14b载体简介The pET‐14b vector (Cat. No. 69660‐3) carries an N‐terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is
3、 reversed on the circular map. The cloning/expression regionof the coding strand transcribed by T7 RNA polymerase is shown below.pET14b载体序列ORIGIN1TTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAATGCGGTAGTTTATCACAGTTAAA61TTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGG121CACCGTCACCCTGGATG
