蚕蛹粗提浸液致敏小鼠哮喘模型的建立.pdf

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1、·162·免疫学杂志2014年2月第30卷第2期IMMUNOLOGICALJOURNALVo1．30No．2Feb．2014·论著·【文章编~-]1000-8861(2014)02-0162-04；FDO~IO．13431~．enki．immuno1．j．20140035蚕蛹粗提浸液致敏小鼠哮喘模型的建立李维中，詹政科，杨成彬，刘志强，肖小军，龚苗，刘志刚【摘要】目的建立蚕蛹粗提浸液致敏Balb／c小鼠哮喘模型，检测分析蚕蛹粗蛋白致敏性。方法以Balb／c小鼠为模型实验动物，分为阴性组、阳性组。阴性组腹腔

2、皮下注射生理盐水；阳性组腹腔注射蚕蛹粗提浸液致敏，连续3次，每次间隔1周。然后滴鼻激发l周每天1次。末次激发后24h内进行气道高检测，收集支气管肺泡灌~(BALF)并对细胞进行分类、计数。检测特异性抗体(IgE)、细胞因子(IL-4、IL一10、INF叫)以及肺组织病理变化等指标对模型的构建进行评价。结果阳性组的小鼠肺部病理改变明显，呈明显炎症病理改变，灌洗液中细胞计数明显升高，嗜酸性粒细胞明显升高(P<0．O1)，灌洗液IL-4、IL一1O升高，INF叫降低；阴性组各项指标均无升高。结论蚕蛹粗提浸液能使

3、Balb／c小鼠发生致敏性哮喘，且致敏性强，建模成功，为以后相关过敏疾病防治奠定科研实验基础。【关键词1蚕蛹；过敏原；肺泡灌洗；哮喘【中图分类号】R392．8．【文献标识码】AEstablishmentofanasthmaticmousemodelsensitizedbysilkwormchrysaliscrudeextractionLIWeizhong，ZHANZhengke，YANGChengbin，LIUZhiqiang，XIAOXia~un，GONGMiao，LIUZhigang*Imtit~eo

4、fAllergyandImmunology,CollegeofMedwine,ShenzhenUniversity,Shemhen518060,China*CorrespondingAuthor：LIUZhigang,E-mail．"lzg@szu．edu．crt[Abstract]TodevelopananimalmodelofasthmainBalb／cmicesensitizedbysilkwormchrysaliscrudeextraction，andtoassaytheallergenicity

5、ofsilkwormchrysaliscrudeextraction，Balb／cmiceastheexperimentalanimalmodelweredividedintonegativegroupandpositivegroup．Negativegroupacceptedintraperitonealinjectionofphysiologicalsaline，whilepositivegroupacceptedintraperitonealinjectionofsilkwormchrysalisc

6、rudeextractforsensitization，continuous3times，withanintervalof1week．Andthen，wedetectedairwayhyperresponsiVenesswithin24hoursafterthelastinspire，andcollectedbronchoalveolarlavagefluid(BALforcellsortingandcounting．Furthermore，weassayedthelevelsofspecificanti

7、bodies(IgE)andcytokines(IL一4，IL一10，theINF一inserumandthepathologicalchangesoftheultrastructureoflungtissue，forevaluatingthemodelbuilding．Resultindicatedthatthepositivegroupmicedemonstratedpulmonarypathologicalchanges(obviousinflammatorypathologicalchanges)

8、；furthermore，inlavagefluidofpositivemice，thecellcounting，eosinophilicgranulocytelevel<0．01)，andtheIL-4andIL-10levelswereallincreased，butINF-~／levelreduced．Whiletheindicatorsofnegativegrouphadnochanges．Inconclusion，a