神经病理性疼痛脊髓背角GLT-1及GLAST表达的变化.pdf

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1、·150·中国疼痛医学杂志ChineseJournalofPainMedicine2014，20(3doi：10．3969~．issn．1006-9852．2014．03．006神经病理性疼痛脊髓背角GLT-1及GLAST表达的变化罗裕辉张德仁(广东医学院附属南山医院，深圳518052)摘要目的：观察神经病理性疼痛大鼠脊髓背角GLT-1及GLAST表达变化对疼痛的影响。方法：选取雄性SD大鼠80只随机分为4组，A组=10)为k背根切断假手术组，B组(7z=30)为k背根切断组，c组(7z=10)为坐骨神经周围植管与药

2、物溶剂组，D组=30)为坐骨神经周围植管与给药组。通过机械刺激的行为学观察确立病理性神经痛形成过程，采用免疫组化进行细胞定量。结果：A及C组胶质细胞GLT-1及GLAST表达无变化。B、D组痛敏形成后脊髓背角胶质细胞GLT-1及GLAST的胞内外表达未发生改变；B及D组小胶质细胞GLAST术后4d表达增加至7d后逐渐下降，而星型胶质细胞GLT-1术后6d表达明显增加，直至14d仍持续增高。结论：脊髓背角胶质细胞GLT-1及GLAST不存在胞吞现象，小胶质细胞参与启动病理性神经痛但不维持其发展，星型胶质细胞主要维持病理

3、性神经痛发展，TNF．仪介导病理性神经痛大鼠脊髓背角GLT-1及GLAST表达并诱导胶质细胞参与和维持病理性神经痛。关键词神经病理性疼痛；肿瘤坏死因子．；脊髓背角；谷氨酸转运体EXPRESSIONOFGLT．1ANDGLASTINSPINALCORDDORSALHoRNWITHNEURoP．ATHICPAINLUOYu．Hui，ZHANGDe．Ren△(AfiliatedNanshanHospitalofGuangdongMedicalCollege，Shenzhen518052)Abstract0bjective：

4、ToobservetheefectoftheexpressionofGLT-1andGLASTinspinalcorddorsalcordwithneuropathicpain．Methods：EightymaleSDratswererandomlydividedintofourgroups．GroupA=10)wastreatedwithshalTloperationwithL5dorsalroottransection，groupB(7z=30)underwentL5dorsalroottransection，g

5、roupC=10)receivedintubationandmedicinesolventectionaroundthesciaticnerveandgroupDreceivedintubationaroundthesciaticnerveandTNF一administration．Mechanicalstimulationwasadaptedtoestablishtheformationprocessofneuropathicpainandimmunohistochemistrywasappliedtoquanti

6、fytherelatedsubstancesandcells．Results：NochangesappearedintheexpressionofGLT-1andGLASTingroupsBandDafterneuropathicpainWasestablished．TheexpressionofGLT-1inrnicroglialcellsincreasedat6claysafteroperation，andcontinuedtoincreasethrough14daysingroupsBandD．GLASTofa

7、strocytesshowedincreasedexpressionat4claysafteroperationuntilthe7clayswhentheexpressionbegantodecreasegradually．TherewerenochangesofGLT-IandGLASTintheglialcellsingroupsAandC．Conclusion：ThereisnoendocytosisphenomenonofGLT-1andGLASTinglialcells．Themicroglialcells

8、areinvolvedintheinductionofneuropathicpainbutdonotsustainthepaindevelopment，andtheastrocytesplayaroleinthesustainmentofpain．TNF—O／．mediatestheexpressionofGLT-1andGLASTinspin