牛栏山二锅头酒醅中芽孢杆菌分离鉴定及发酵风味分析.pdf

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1、研究摆{寸Fo1．33，No．09，2012午枉山二锅头酒醅著兆讦菌分离鉴足&反踌风味分祈杨春霞’，廖永红，刘峻雄’，胡建华，胡佳音，窦岫’(1．北京工商大学食品学院，食品添加剂与配料北京市高等学校工程中心，食品风味化学北京市重点实验室，北京100048；2．北京顺鑫农业股份有限公司牛栏山酒厂，北京101301)摘要：从牛栏山二锅头酒醅中分离筛选出5株产风味物质能力较好的芽抱杆菌，通过16SrDNA序列分析和构建系统发育树．5株细菌分别为地衣芽孢杆菌(Bacilluslichenrmis)、蜡样芽孢杆菌(Bac

2、illuscel'e~s)、短小芽孢杆菌(Bacillusp“milus)和枯草芽孢杆菌(Bacillussubtilis)分别对它们进行发酵风味分析，其发酵液经固相微萃取和GC—MS分析．并除去空白培养基中物质，地衣芽孢杆菌BL—l发酵液共检测得到14种风味物质，蜡样芽孢杆菌BC一1和短小芽孢杆菌BP一1发酵都得到12种风味物质，枯草芽孢杆菌BS—l好氧发酵共得到16种风味物质，枯草芽孢杆菌BS一2厌氧发酵共得到l9种风味物质、除短小芽孢杆菌外，其他4株芽孢杆菌都含有较多数量的酯类化合物，且主要代谢风味物质都

3、是3一羟基一2一丁酮，而短小芽孢杆菌BP一1则含有数量较多的烃类化合物，其主要风味物质是苯乙醇。关键词：风味物质，酒醅，固相微萃取，气相色谱一质谱联用仪，芽孢IdentificationofBacillusfrOmNiulanshanErguotoufermentedgrainandanalysisofflavorcompoundsinthefermentationYANGChun-xia，LIAOYong-hong．’，LIUJun-xiong。，HUJian-huaz,HUJia-yinz,DOUShen’

4、(1．SchoolofFoodandChemicalEngineering，BeijingHigherInstitutionEngineeringResearchCenterofFoodAdditivesandIngredients，BeijingKeyLaboratoryofFlavorChemistry，BeijingTechnologyandBusinessUniversity，Beijing100048，China：2．NiulanshanDistillery，BeijingShunxinAgricul

5、tureCo．，Ltd．，Beijing101301，China)Abstract：FivestrainsOfbac⋯USwhichcanproduceflavorwerescreenedfrOmNiulanshanErguotoufermentedgrainUsingthesequencesanalysisof16SrDNAandphylogenetictreeconstruction．fivestrainswereidentifiedasBacillus／icheniformis．Baci／／uscereu

6、s．BacilluspumilusandBacillussubtilisThefermentationbrothOffivebacillusstrainswereanalyzedbysolidphasemicro—extractionandchromatography—massspectrometryRemovingthecompoundsofblank．atotaIOf14flavorcompoundsinfermentationbrothofBacillusfichenifom1fsBL一1．12flavo

7、rcompoundsinfermentationbrothofBacilluscereusBC一1andBacilluspumilusBP一1，16flavorcompoundsinfermentationbrothofBacillussubtilisBS一1．19flavorcompoundsinfermentationbrothofBacillussubtilisBS一2．ExceptBaciluspumilus．thefermentationofotherfourBacillusstrainsmainly

8、containedesterscompound．and3一hydroxy一2一butanonewasthemostimportantflavorcompoundHowever，thefermentationofBaciluspumilusBP一1mainlycomprisedalkynescompoundandphenylethylalcoholwasthemainflavorcomp