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时间:2020-06-03
《日本沼虾卵巢发育的组织学与组织化学观察.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、广东农业科学2014年第13期119日本沼虾卵巢发育的组织学与组织化学观察杜玉昕,邱高峰(上海海洋大学农业部水产种质资源与利用重点开放实验室,上海201306)摘要:应用组织切片、组织化学技术,对日本沼虾雌性生殖系统的组织结构、卵巢发育周期进行研究。结果表明,日本沼虾雌性生殖系统由卵巢和输卵管组成,卵巢外被结缔组织膜与生殖上皮。日本沼虾的卵巢发育具有明显的色泽与体积的变化,根据其外部形态和内部结构,将其划分为增殖期、小生长期、大生长期、成熟期和恢复期5个时期。卵子发生可分为卯原细胞期、卵黄发生前期、卵黄发生期、卯黄发生后期4个时期。卵黄发
2、生前期的卯母细胞为小生长期的初级卯母细胞,胞质嗜碱性,无卵黄颗粒;卵黄发生期卯母细胞为大生长期卵母细胞,胞质嗜酸性,早期含卵黄颗粒,后期转变为卵黄块,核位于卵母细胞中央;卵黄发生后期卵母细胞为成熟期卵母细胞,体积长足,核移动到卵母细胞周边,最终核生发泡破裂,核膜消失,卯周围滤泡细胞层变得极薄,等待排卵。关键词:日本沼虾;雌性生殖系统;组织学:组织化学中图分类号:$917:Q952.1文献标识码:A文章编号:1004—874X(2014)13-01l9—05Histologicalandhistochemicalobservation0no
3、varydevelopment●●l●l,l■●●inorientalrlverprawnMacroOraC,zZumnZ矽矽0nenDUYu-xin,QIUGao-feng(KeyLaboratoryofAquaticGeneticResourcesandUtilizationCertificaredbyMinistryofAgricuhure,ShanghaiOceanUniversity,Shanghai201306,China)Abstract:Inthestudy,histologicsectionandhistochemica
4、lmethodswereusedtoresearchtheorganizationalstructureoffemalereproductivesystemandovarydevelopmentcycleinorientalriverprawnMacrobrachiumnipponense.Theresultsshowthatthefemalereproductivesystemiscomposedofovaryandoviduct,andtheovaryiscoveredwithconnectivetissuecapsuleandrep
5、roductiveepithelium.Theovariandevelopmentoforientalriverprawnhassignificantchangesincolorandvolume,therefore,accordingtotheexternalshapeandinternalstructure,ovariandevelopmentcanbedividedintofivestages,includingmultiplicationstage,minorgrowthstage,majorgrowthstage,maturat
6、ionstageandrecoverystage.Oogenesiscanbedividedintofourstages:oogoniumstage,previtel10genesisstage,vitellogenesisstage,latevitellogenesisstage.Theprevitellogenesisoocytesisregardedasnichelong—termprimaryoocyte,thecytoplasmicisfilledwithbasophiliccellsandnoyolkgranules.Thev
7、itellogenesisoocytesisthehugegrowingoocytes,thecytoplasmicisfilledwitheosinohiliccells,theearlyvitellogenesisoocytescontainsyolkgranulesandblockslateintoyolk,thenucleusislocatedinthecentralofoocytes.Theperiodoftheoocyteshasbeenmaturewithgreatvolumeinthelatevitellogenesiss
8、tage,nuclearmovestothesurroundingoftheoocyte,eventuallythegerminalvesiclebreakdownsandthenuclear
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