成年SD大鼠糖尿病心肌病模型的建立.pdf

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1、中国当代医药2014年8月第21卷第23期论著·成年SD大鼠糖尿病心肌病模型的建立张良胜李平，许翔1王莉范忠才1．河南省永城市人民医院心内科，河南永城476600；2．泸州医学院附属医院心内科，泸州646000【摘要】目的探索成年SD大鼠糖尿病心肌病(DCM)模型建立的方法。方法2O只健康SD成年大鼠作随机分为DCM组(lo)、对照组(=lo)。对照组喂养普通饲料，DCM组高脂饲料喂养12周。DCM模型的建立：对照组以枸橼酸缓冲液腹腔注射，DCM组使用等量1％链脲菌素腹腔注射。通过病理组织学证实为DCM心肌病理学改变。结果与对照组比较，

2、DCM组的血清FBG、TC、LDL—C、TG均明显增高(P<0．05)，而对照组的血清HDL—C较DCM组高(P<0．05)。与对照组比较，DCM组的大鼠心肌细胞排列紊乱，间质基质集聚与纤维化增多。第6周末，两组的空腹血糖差异无统计学意义(尸>0．05)，DCM组的IRI、胰岛素均高于对照组(P

3、4EstablishmentofdiabeticcardiomyopathyofadultSpprague-dawleyratmodelZHANGLiang-shenfLIPgXUXiandWANGLi

4、FANZhong-cai21．DepartmentofCardiology，YongchengPeopleSHospitalofHenanProvince，Yongcheng476600，China；2．DepartmentofCardiology，AffiliatedHospitalofLuzhouMedicalCollege，Luz

5、hou646000，China【Abstract】ObjectiveToexplorethemethodhowtoestablishofadultSpprague—dawleyratmodelofdiabeticcar-diomyopathy(DCM)．Methods20healthyadultSpprague—dawleyratswererandomlydividedintotheDCMgroup=lo)andthecontrolgroupfn=lO)．RatsinthecontrolgroupandDCMgroupwasrespec

6、tivelyfedwithnormalratdietandhighfatdietpersistently12weeks．STZwasappliedintheDCMgrouptomaketheratDCMmodelbyintraperi-tonealinjection，andthesamedosageofcitricacidbufferwereusedtothethecontrolgroupbythesameway．Themy—ocardialpathologicalalterationofDCMwasidentifiedintheDCM

7、groupbyhistopathology．ResultsComparedwiththecontrolgroup，fastingbloodglucose(FBG)，low-densitylipoproteincholesterol(LDL-C)，theserumtotalcholesterolc1rC)，andtriglyeride(TG)ofratsintheDCMgroupwerehigher(P<0．05)，theserumHigh-densitylipoproteincholesterol(HDL-C)inthecontrolg

8、roupwashigerthanthatintheDCMgroup(尸<0．05)．Comparedwiththecontrolgroup，ratmy—ocardialcellsoftheDCMgrouparrangedindisorder,interstitialmatrixaccumulationandfibrosisincreased．Attheendof6week，therewasnostatisticaldifferenceoffastingbloodglucoseinthetwogroups(P>0．05)，butinsul

9、inandinsulinresistanceindexintheDDMgroupwerehigherfP≮0．05)．ConclusionEstablishmentofDCMmodelbyintraperi