影响麻疯树叶片离体再生条件的优化.pdf

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1、2014年1月四川大学学报(自然科学版)Jan．2014第51卷第1期JournalofSichuanUniversity(NaturalScienceEdition)Vo1．5lNo．1doi：103969／j．issn．0490—6756．2014．01．36影响麻疯树叶片离体再生条件的优化朱雅雅，范林洪，王胜华，陈放(四川I大学生命科学学院生物资源与生态环境教育部重点实验室，成都610064)摘要：为建立高效稳定的麻疯树离体再生体系，优化了诱导和分化培养基及培养条件．以无菌苗叶片为外植体，以MS为基本培养基，6-BA2．0m

2、g／L+2，4一D0．02mg／L的激素组合对愈伤组织的诱导最有利，6-BA0．5mg／L+KT0．5mg／L+IBA0．1mg／L+GA。0．1mg／L的激素组合对不定芽分化最有利．在此基础上针对外植体培养、愈伤组织继代的培养条件和继代次数3个因素进行优化．结果表明：采用麻疯树的无菌苗叶片为外植体，黑暗条件下诱导出愈伤组织，再在黑暗条件下继代一次后转入分化培养基中对不定芽诱导最为有利，分化率达72．59．分化后得到的不定芽在含IBA0．2mg／L的MS培养基上生根率达75％．生根苗在添加蛭石的含IBA0．05mg／L的改良生根培

3、养基中驯化培养后，再经过以蛭石：腐殖土一1：l(V／V)为培养基质的套袋培养，移栽后成活率达93．关键词：麻疯树；叶片；继代；芽再生；优化中图分类号：Q949．7文献标识码：A文章编号：0490—6756(2014)01—0213—06OptimizationoffactorsaffectinginvitroadventitiousshootregenerationfromleavesofJatrophacurcasL．ZHUYa—Ya，FANLin—Hong，WANGSheng—Hua，CHENFang(KeyLaborator

4、yofBio—sourcesandEco—environmentoftheMinistryofEducation，CollegeofLifeSciences，SichuanUniversity，Chengdu610064，China)Abstract：ToestablishasystemofstableandefficientinvitroregenerationofJatrophaCurcasL．，anoptimizedinductionanddifferentiationmediumandcultureconditionswe

5、reexplored．TheleavesfromsterileseedlingsofJatrophaCUrCaSL．wereselectedasthebestexplants．MurashigeandSkoog(MS)mediumcontaining2．0mg／Lhenzyladenine(6一BA)and0．02mg／L2，4-dichlorophenoxyaceticacid(2，4一D)waschoosedforcallusinductionandMSmediumcontaining0．5mg／L6一BA，0．5mg／Lki

6、netin(KT)，0．1mg／Lindole一3一butyricacid(IBA)and0．1mg／Lgibberellins(GA3)wasadoptedasmoreap—propriatemediumforadventitiousbudsregeneration．Onthisbasis，threefactorsincludingexplantcul—tureconditions，sub—cultureconditionsofcallusandsub—culturetimeswerefurtherstudiedandopti—

7、mized．Theresultssuggestedthatcalluswereinducedfromleavesofsterileseedlingsindarkconditions．Andthenthesecallusweresub-culturedforonetimeinthesameconditions，about72．59frequencyofshootregenerationwasobtained，SOthedarkconditionsforcertaintimewassuitablefortheadventitiousb

8、udsregeneration．Therootingpercentageofthedifferentiatedadventitiousbudsreachedupto75％onMScontaining0．2mg／LIBA．Approximately9