电针结合磁刺激对脊髓损伤大鼠逼尿肌兴奋性及α-SMA的影响.pdf

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1、中国康复·2013年1O月·第28卷第5期327电针结合磁刺激对脊髓损伤大鼠逼尿肌兴奋性及一SMA的影响程宇核，王俊华，李海峰，朱小虎，谢谨，邹旭丹，万超【摘要】目的：探讨电针及磁刺激治疗脊髓损伤(SCI)模型大鼠，对其逼尿肌兴奋性及a一平滑肌肌动蛋白(a—SMA)的影响，从而探讨其作用机制。方法：SD大鼠5O只，随机分为正常组、模型组、电针组、磁刺激组和联合组，分别采用对应的治疗；采用重物坠落打击方法制备SCI模型；治疗后，测定各组大鼠离体逼尿肌肌条的最小收缩张力及收缩频率，采用免疫组化法观察大鼠逼尿肌中“一sMA

2、阳性细胞数量。结果：治疗1O次后，膀胱逼尿肌离体肌条最小收缩张力比较，联合组明显低于模型组、磁刺激组和电针组，电针组和磁刺激组均明显低于模型组(均P<0．05)；各组肌条收缩频率及n—SMA阳性细胞表达比较，联合组均明显高于模型组、磁刺激组和电针组，电针组和磁刺激组均明显高于模型组(均P

3、号】R49；R683．2[DOII10．3870／zgkf．2013．05．002EffectofelectrOacupuncturecombinedwithmagneticstimulationtherapyontheexcitabilityofdetrusorandexpressionofSMAinratswithspinalcordinjuryCHENGYu—he，WANGJun—hua，LIHai—feng，eta1．A{{tliatedTaiheHospitaltoHubeiUniversityofMedi

4、cine，Shiyan442000，China[Abstract]Objective：Toinvestigatetheinfluenceofelectroacupunctureandmagneticstimulationtherapyonthedetrusorexcitabilityanda—SMAofratswithspinaIcordinjury(SCI)andthemechanism．Methods：FortySDratswererandomlydividedinto5groups：normalgroup，mo

5、delgroup，electroacupuncturegroup，magneticstimulationgroup，andcombinationgroup．TheSCImodelwaspreparedbyheavyobjectsfallmethod．Theminimumshrinkagetensionandcontractionfrequencyofisolateddetrusormusclestripsinratsweremeasured．Immunohistochemicalmethodswereadoptedt

6、oquantifythedSMApositivecellsofthedetrusor．Results：After10timesoftreatment。theminimumshrinkagetensionincombinationgroupwasobviouslylowerthanmodelgroup，e1ectroacupuncturegroupandmagneticstimulationgroup，andthatinelectroacupuncturegroupandmagneticstimulationgroup

7、waslowerthaninmodelgroup(allP<0．05)．Thecontractionfrequencyandthenumberofa—SMApositivecellsincombinationgroupwereobviouslyincreasedascomparedwithmodelgroup，electroacupuncturegroupandmagneticstimulationgroup，andthoseinelectr0acupuncturegroupandmagneticstimulatio

8、ngroupweresignificantlyincreasedascom—paredwithmodelgroup(allP