蜂王浆主蛋白（MRJPs）超滤分离工艺优化及产物质量分析.pdf

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1、浙江大学学报农业与生命科学版405526~5322014JournalofZhejiangUniversityAgric&LifeScihttpwwwjournalszjueducnagrE-mailzdxbnsb@zjueducnDOI103785jissn1008-9209201402131蜂王浆主蛋白MRJPs超滤分离工艺优化及产物质量分析李玫璐11221*谭量量裘卫尹志红沈立荣1浙江大学生物系统工程与食品科学学院馥莉食品科学研究院杭州3100582杭州碧于天保健品有限公司杭州311500摘要采用超滤方法从鲜王浆中提取王浆主蛋白majorr

2、oyaljellyproteinsMRJPs先就水料比、pH、离子强度3种单因素对MRJPs提取率的影响进行单因素分析然后通过正交试验对MRJPs的超滤提取工艺进行优化最后对MRJPs分离产物冻干粉和滤后液进行成分测定结果表明最佳提取工艺为水料体积质量比5∶1mLgpH7.0离子强度0.5molL在最佳工艺条件下MRJPs的提取率为82.63%冻干粉含可溶性蛋白91.00%、水分0.01%、总糖15.22%、王浆酸2.00%超滤副产物———滤后液中王浆酸和总糖的回收率分别为3.60%和2.11%超滤工艺具有无溶剂污染、王浆成分可回收利用适合规模化

3、生产的优点为MRJPs分离提取提供了新的工艺方法为王浆深加工和MRJPs蛋白产品开发提供了科学依据关键词蜂王浆王浆主蛋白超滤分离正交试验中图分类号S896.3文献标志码AOptimizationofultrafiltrationseparationandqualityanalysisofthemajorroyaljellyproteinsJournalofZhejiangUniversityAgric&LifeSci201440552653211221*(1FuliInstituteofFoodScienceCollegeLiMeiluTanLi

4、angliangQiuWeiYinZhihongShenLirongofBiosystemsEngineeringandFoodScienceZhejiangUniversityHangzhou310058China2HangzhouBiyutianHealthFoodCoLtdHangzhou311500China)SummaryRoyaljellyRJistheexclusivefoodfortheWesternhoneybeeApismelliferaqueenthroughoutitslifespanandtheprimaryfoodfo

5、rthehoneybeelarvaeduringthefirstthreedaysoflifewhichissecretedbyhypopharyngealandmandibularglandsofnursehoneybeesThechemicalcompositionofRJisverycomplicatewhiletheproteincomponentshavebeenfoundtoplaycriticalrolesinRJAround50%ofdryRJismadeupofproteinswhichcouldbedividedintotwo

6、partswater-solubleproteinsandwater-insolubleproteinsThewater-solubleproteinsaccountingfor82%to90%ofthetotalRJproteinsarenamedmajorroyaljellyproteinsMRJPswhichbelongtothesamefamilycomposedofninemembersIthasbeenprovedthatMRJPshaveeffectssuchasanti-agingimmunoregulationandanti-f

7、atigueetalInadditionMRJPsespeciallyMRJP1areauthenticatedasthecriticalconstituentstorevealthefreshnessofRJandwillbehopefulasonenewfunctionalcomponentinfutureInpreviousstudiesvariousmethodsweredevelopedforisolationofMRJPswhichincludedcentrifugationalkaliextractionacidprecipitat

8、ionandcolumnchromatographyHoweveraseriesofobstaclessuchaslowpurity基金