捏脊疗法对脾虚大鼠学习记忆及其皮层BDNF和GDNF阳性细胞数目的影响.pdf

《捏脊疗法对脾虚大鼠学习记忆及其皮层BDNF和GDNF阳性细胞数目的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、第9卷第5期中医儿科杂志Vo1．9No．52013年9月J．PEDIATRICSOFTCMSept2013·实验研究·捏脊疗法对脾虚大鼠学习记忆及其皮层BDNF和GDNF阳性细胞数目的影响李硕，张同园(天津中医药大学第二附属医院儿科，天津300153)摘要：目的观察捏脊疗法对脾虚大鼠学习记忆及其大脑皮层脑源性神经生长因子(BDNF)和胶质源细胞性神经营养因子(GDNF)阳性细胞数目的影响，并探讨其作用机制。方法通过建立利血平致脾虚动物模型，设立正常组、模型组、阳性对照组、捏脊组。测定大鼠体质量、学习记忆和游泳时间等指标，评定脾虚反应的强度。通过免疫

2、组织化学技术，研究各组大鼠大脑皮层BDNF和GDNF阳性细胞数目的变化。结果捏脊疗法使脾虚大鼠的体质量明显增加的同时，游泳时间明显延长(P

3、)05—0008—04EfectofchiropractictherapyOillearningandmemoryandthenumberofcortexBDNFandGDNFpositivecellsofspleendeficiencyratsLiShuo，ZhangTongyuan(PediatricDepartmentoftheSecondAffiliatedHospitalofTianjinUniversityofTCM，Tianjin，300153，China)Abstract：ObjectiveToobservetheeffectofc

4、hiropractictherapyonspleendeficiencyratlearningandmemoryaswellasthenumberofpositivecellsofbraincortexnervegrowthfactor(BDNF)andglialcellsderivedneurotrophicfactor(GDNF)，andexploreitsmechanism．MethodsAfterestablishmentofreserpineinducedspleendeficiencyanimalmodel，theratswerediv

5、idedintonormalgroup，modelgroup，positivecontrolgroup，chi—ropractictreatmentgroup．Determinebodyweight，learningandmemory，swimmingtimeandotherindexestoevaluatetheintensityofthereactionofspleendeficiency．Byimmunohistochemistry，toinvestigateBDNFandGD-NFpositivecellnumberofcerebralco

6、rtexineachgroup．ResultsComparedwithothergroups，bodyweightandswimmingtimeinchiropracticgroupincreasedsignificantly(P

7、bralcortexsignificantlyreduced(P<0．05)．ConclusionChiropractictherapycaneffectivelyimprovelearningandmemoryabilityofspleendeficiencyrats，anditsmechanismmayberelatedtocor—texBDNFandGDNFpositivecellproteinexpressionleve1．Keywords：chiropractictherapy；spleendeficiency；learningandme

8、mory；BDNFpositivecells；GDNFposi—tivecells；experimentalstudy脾虚