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ID:55612864
大小:1.48 MB
页数:4页
时间:2020-05-20
《线粒体途径在大黄素促人混合培养淋巴细胞凋亡中的作用.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、现代肿瘤医学2015年06月第23卷第11期MODERNONc0L0GY,Jun.2015,VOL23,NO.11·1495·线粒体途径在大黄素促人混合培养淋巴细胞凋亡中的作用沈乃营,刘昌,何盟国,曲凯,张天政EfectofthemitochondrialsignalingpathwayonEmodin——inducedapoptosisinhumanmixedlymphocytecultureShenNaiying,LiuChang,HeMengguo,QuKai,ZhangTianzhengDepartmentofHepatobiliarySurge
2、ry,ShaanxiXianyang215Hospital,ShaanxiXi(myang712000,China;DepartmentofHepatobiliarySurgery,theFirstAffiliatedHospitalMedicalSchoolofnJiaotongUniversity,ShaanxiXihn710061,China.【Abstract】0bjective:ToinvestigatetheeffectofmitoehondrialsignalingpathwayonEmodin—inducedapoptosisinhuma
3、nmixedlymphocyteculturemode1.Methods:Themodelofhumanmixedlymphocyteculturewasestablishedandlymphocyteswereisolatedfromtheperipheralbloodsamplesofdifferenthealthyadultvolunteers.ToinvestigatetheimmunosuppressiveeffectofEmodin.Emodinwasadministeredwithdifferentconcentrationsat1i.~m
4、ol/L,101~mol/L,1001~mol/Lfor24,48,72h.Attheendofculture,theapoptoticrateoflymphoeytesandthelevelofreactiveoxygenspecies(ROS)andmitoehondrialmembranepotential(△m)wereevaluatedwithfloweytometry.Proteinexpres-sionsofeytosoliccytochromeC,caspase一3andcaspase一9wereanalyzedbyWesternblot
5、.InthehighconcentrationEmodingroup,lymphocyteswereculturedwithreducedglutathione(GSH)andEmodinfor48h,GSHcanremovein-tracellularROS.TheapoptoticrateoflymphocytesandthelevelsofROSand△蛳werealsoevaluatedbyfloweytom-etry.ProteinexpressionsofBcl一2andBaxwereanalyzedbyWesternblot.Results
6、:WiththeenhancedconcentrationofEmodin,theinhibitionofproliferationandincreaseofapoptoticratewereobserved.TheresultsfurthershowedgenerationofROS,disruptionof△m,decreaseoftheBcl一2/BaxratioandcytosoliccytochromeC.Caspase一3andcaspase一9levelsalsoincreasedaftertheEmodintreatment.Thedec
7、lineintheBcl一2/BaxratioandtheincreaseofROS,cytosoliccytochromeCandcaspase一3andcaspase一9levelswereconsistentwiththeincreaseofthelympho—cyteapoptoticratio.IntheGSHgroup,thelevelofROSdeclined,andtheapoptoticratealsoreducedcomparedwiththehighconcentrationEmodingroup(P<0.05),however,t
8、heAgtmenhancedcomparedwithcontrolgroup(P
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