鼠疫血清抗体谱的研究进展.pdf

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1、地方病通报2008年第23卷第5期EndemicDiseasesBulletin2008Vol,23，No,5·79·文章编号：1000—3711(2008)05-0079-04InvestigativeProgressesofPlaguePatientSerumAntibodyProfile*ZHOUQing，SONGZhi—zhong2一$(SchoolofPublicHealth，DaliUnwemity，Dali，Yunnan671000，China)Abstract：ObjectiveUsingthetechniqueofproteinc

2、hiptoexamtheplaguegermsandanalyzetheplaguegermserumantibodyprofile．MethodsThepreparedproteinchipwasdetectedbyfluorescenceandthedatawereanalyzedafterhybridizedwithunknownplaguegermserum．ResultsGAP—associatedproteinwasfoundwithantigenicityandimmunogenicity．ConclusionAccordingtot

3、heanalysisoftheserumantibodyprofilewecangettheimmunogenicproteinwhichcanbeusedasthecandidatevaccinegroup，thisisthefirstprofits．Secondlywecanalsogettheantigenwhichcanassistthediagnosisofplaguegerminfection．FinaUythroughtheanalysisofthechangingtendencyoftheseriesserumantibodiesa

4、ndthediferencesamongthean—tibodvprofileofdiferentspecies，wecandeepenourknowledgeaboutplaguegermpathogenesismechanism．Keywords：Plague；Proteinchip；AntibodyprofileCLCnumber：R316．8；R一1；Documentcode：A鼠疫血清抗体谱的研究进展(摘要)周青，宋志忠(大理学院公共卫生学院，云南大理671000)摘要：目的利用蛋白芯片技术对鼠疫血清进行检测，分析其血清抗体谱。方法用制备

5、好的蛋白芯片和未知的血清进行杂交后荧光检测并数据分析。结果发现具有抗原性，免疫原性的相关蛋白。结论通过分析血清抗体谱而得到新的具有免疫原性的蛋白作为潜在的候选疫苗组；得到新的可以用于鼠疫菌诊断的抗原，作为目前血清学检测的辅助诊断抗原；通过对系列血清抗体变化趋势以及不同种属间抗体谱的区别的分析，加深对鼠疫菌致病机制的认识。关键词：鼠疫；蛋白芯片；血清抗体谱PlagueisthestronglyinfectivediseasetransmittedbyvelopingfirstDNAchipintheworldin1991，andthisfleaand

6、itisquitepopularintherodent．Therehavesuccessraisedthecurtainforstudyingthebiochiptech—beenalreadythreeprevalencesintheworldfromthe6thnologyfromthenon．Nowadays，DNAchiptechnologyiscenturytotheendofthe19th．Plaguecanbedividedintoappliedtothedetectionandcomparativestudyonvarious,gl

7、andplague，pulmonaryplagueandsepticemiaplaguenucleicacidarraysexpression，nomatterthebiologicalaccordingtotheclinicalsymptoms．Glandbubonicplaguesampleisknownornot[2】．However，itcannotshowanyhavethetypicalclinicalmanifestationscomparedwithdirectrelationshipbetweentheproteinswhichh

8、asfunc—pulmonaryplagueandsepticemiaplague，butthelattersfioned