痉挛型瘫痪大鼠骨骼肌DNA甲基化水平与肌纤维构型.pdf

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1、中国康复理论与实践2015年5月第21卷第5期ChinJRehabilTheoryPract，May，2015，Vo1．21，No．5—519一DOI：10．3969~．issn．1006-9771．2015．05．006·基础研究·痉挛型瘫痪大鼠骨骼肌DNA甲基化水平与肌纤维构型王玉昌，庞伟，冯欢欢，刘师振，张慧【摘要l目的探讨骨骼肌在痉挛状态下对DNA甲基化水平的影响及与肌纤维构型的相关性。方法健康5日龄Wistar新生仔鼠100只，随机分为模型组和对照组。前者制备痉挛型瘫痪大鼠模型成功后饲养30d。对两组大鼠腓肠肌进行肌肉活检。分别进行骨骼肌DNA甲基化水平测定、骨骼肌I型肌球蛋白重链

2、mRNA半定量RT-PCR检测，透射电镜观察。结果模型组骨骼肌总体DNA甲基化水平(4．95~0．83)x10％，显著低于对照组的(6．59~0．75)x10％(P<0．OO1)；模型组骨骼肌I型肌球蛋白重链mRNA表达量(1．23~0．31)，显著高于对照组的(0．44~0．29)(p

3、构型改变相关。【关键词】痉挛型瘫痪；甲基化；超微结构；I型肌球蛋白重链DNAMethylationandMuscleFiberConfigurationonSkeletalMuscleinSpasticParalysisRatsWANGYu—changi．PANGWeil．FENGHuan—huan1．LIUShi-zhen2,ZHANGHui31．RehabilitationMedicalResearchInstituteofJiamusiUniversity,Jiamusi，Heilongjiang154002，China；2．Shan—dongJiningNo．1People’sHos

4、pital，Jining，Shandong272000，China；3．YanzhouTieluHospital，Jining，Shandong272100，ChinaAbstract：0bjecfiveToinvestigatetheDNAmethylationofskeletalmuscleinspasticparalysisratsandcorrelationwiththemusclefi．berconfiguration．Methods1005-dayoldWistarratswererandomlydividedintomodelgroupandcontrolgroup．Thefo

5、rmerwasestab—lishedthespasticparalysismodleandrearedfor30days．Then，tissuesfromthegastrocnemiusofalltheratswereobservedwithtriplicateDNAmethylation，myosinheavychain-I(MHC-I)mRNAwithRT-PCRandtransmissionelectronmicroscopy．ResultsTheDNAmethyla．tionwas(4．95~0．83)×10％inthemodelgroup，significantlylesstha

6、n(6．59~0．75)×10％inthecontrolgroup(P<0．001)；whiletheMHC-ImRNAwas(1．23~0．31)，significantlymorethan(O．44~0．29)inthecontrolgroup<0．001)．TheZ-linewasdisordered，andthemitochon-drianeartheZ-lineincreased．withedemaandpartiallybrokenincristae．Thebalancebetweenthethickandthinfilamentswasbroken．andmyofibrilse

7、nvelopefused．ConclusionHypomethylationandhyperexpressionOfMHC—ImRNAhavebeenfoundinskeletalmuscleofspas—ticparalysisrats，whichmayresultintypeIfibersincrease．However,therewasnosuficientevidencetosupportthecor