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时间:2020-05-12
《肺炎支原体肺炎患儿血清中IL-9、IL-17水平.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第40卷第7期贵阳医学院学报Vo1.40No.72015年7月JOURNALOFGUIYANGMEDICALCOLLEGE2015.7肺炎支原体肺炎患儿血清中IL一9、IL一17水平何军,张姝,许琦,尹科,张捷,田洪伦,祝静,罗洁(贵州省第二人民医院检验科,贵州贵阳50004)[摘要]目的:检测肺炎支原体肺炎患儿血清中IL一9、IL一17水平,探讨肺炎支原体感染对机体免疫功能的影响。方法:将6O例肺炎支原体肺炎患儿按肺炎支原体抗体不同稀释倍数滴度阳性(1:80、1:160、≥1:320)分为3组作为实验组,2O例正常儿
2、童作为对照组,用酶联免疫吸附试验(ELISA)检测各组血清IL一9、IL.17水平。结果:l:80倍和1:160倍组患儿血清ILO较正常对照降低(P<0.05),各滴度阳性实验组IL·l7水平较正常对照组降低(P<0.05);IL-9随1:80、1:160倍、≥1:320倍滴度阳性逐渐升高[(14.82±5.11)ng/L、(16.374-6.0)ng/L、(18.944-5.86)ng/L、(P<0.05)],而IL-l7水平变化不大[(4.88±2.90)ng/L、(4.71-4-2.38)ng/L、(5.77±1
3、.94)ng/L、(P>0.05)]。结论:肺炎支原体感染可能导致机体血清中IL-9、IL一17水平降低,IL一9水平表现随肺炎支原体抗体滴度升高而升高。[关键词]肺炎支原体肺炎;儿童;肺炎支原体;白细胞介素.9;白细胞介素.17;免疫功能[中图分类号]R375.2[文献标识码]A[文章编号]1000-2707(2015)0443712433Levels0fIL.9andIL.17inSerumofChildrenwithMycoplasmapneumoniaePneumoniaHEJun,ZHANGShu,XUQi,
4、YINKe,ZHANGJie,TIANHonglun,ZHUJing,LOUJie(DepartmentofClinicalLaboratory,SecondPeopleHospitalofGuizhouProvince,GuiyangD0,Guizhou,China)[Abstract]Objective:TodetectthelevelsofIL一9andIL-17inserumofchildrenwithMycoplasmapneumonia(MP)pneumonia(MPP),andsoastoinvestig
5、atetheeffectofMPonimmunefunction.Methods:SixtychildrenwithMPPweredividedinto3groupsaccordingtoMPantibodydilutiontiter(1:80group,1:160groupand≥1:320group),20normalchildrenservedascontrolgroup.LevelsofIL-9andIL-17insertlmweremeasuredbyenzymelinkedimmunosorbentassa
6、y(ELISA).Results:Comparedwiththenormalgroup,thelevelsofIL一9inserumof1:80groupand1:160groupwerede—creased(P<0.05),andthelevelsofIL一17ineachexperimentalgroupweredecreased(P<0.05);thelevelsofIL一9(14.82±5.11ng/L,16.37±6.0ng/L,18.94±5.86ng/L)increasedgraduallywiththe
7、riseofantibodytiters(1:80,1:160,≥l:320),andthedifferencesweresignificant(P<0.05),whiletherewaslittlechangeinlevelsofIL一17(4.88±2。90ng/L,4.71±2.38ng/L,5.77±1.94ng/L).Conclusions:MPinfectionmayleadtothedecreaseofIL一9andIL-17levelsinserum,andthelevelsofIL-9increase
8、withtheincreaseofMPantibodytiter.[Keywords]Mycoplasmapneumoniaepneumonia;children;Mycoplasmapneumoniae;interleukin-9;interleukin一17:immunefunction肺炎支原体(my—coplasma,pn
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