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时间:2020-05-12
《灯盏花素注射液对脂多糖致大鼠急性肺损伤保护作用机制的研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、HainanMedJ,Jan.2015,Vo1.26,No.2海南医学2015年1月第26卷第2期doi:10.3969/j.issn.1003—6350.2015.02.0058灯盏花素注射液对脂多糖致大鼠急性肺损伤保护作用机制的研究杨秋获,李建辉(安新县医院内科,河北安新071600)【摘要】目的探讨灯盏花素注射液对脂多糖致大鼠急性肺损伤的保护作用。方法健康雄性sD大鼠30只随机分为对照组、脂多糖组(LPS组)和灯盏花素干预组(Bre组)各lO只。对照组静脉注射生理盐水0.1ml/kg;LPS组和Bre组静脉注射脂多
2、糖5mg/kg;Bre组在注射脂多糖前后30min分别腹腔注射灯盏花素50mg/kg。观察三组SD大鼠肺组织病理形态学改变及肺组织中超氧化物歧化酶活性(S0D)、丙二醛含量(MDA)及细胞间粘附分子-1OCMA-I)的表达变化。结果与对照组比较,LPS组与Bre组SOD降低(P<0.05)、MDA增多(P3、低细胞间粘附分子一1的表达来减轻脂多糖致大鼠急性肺损伤。【关键词】急性肺损伤;灯盏花素;脂多糖;细胞间粘附分子一1【中图分类号】R-332【文献标识码】A【文章编号】1003-6350(2015)02~0163一o3Protectiveefectsofbreviscapineinjectiononacutelunginjuryinducedbyllpopolysaccharidesinrats.YANGQiu—di,LIJian-hui.DepartmentofInternalMedicine,HospitalofAnx4、inCounty,Anxin071000,Hebei,CHINA【Abstract】ObjectiveToexploretheprotectiveeffectofbreviscapine(Bre)onacutelunginjuryinducedbylipopolysaccharides(LPS)inratsanditspossiblemechanisms.MethodsThirtymaleSprague-Dawleyratswereran—domlydividedintothreegroups:Controlgroup,5、LPSgroup,Breviscapine(Bre)group,eachwith10rats.Controlgroupwasinjectedby0.1ml/kgnormalsaline.LPSgroupandBreviscapinegroupwasinjectedofLPSf5mg/kg).Ad—ditionally,Bregroupreceivedintraperitonealinjectionofbreviscapine(50mg/kg)30minutesbeforeLPSinjectionand30minafter6、LPSinjection.Morphologicalchangesoflungtissueswereobservedunderlightmicroscope.Theac-tivityofsuperoxidedismutase(SOD),thecontentofmalondialdehyde(MDA)inlungtissuesandthecontentoflinter-cellularadhesionmolecule一1(CAM一1)inserumweredetectedineachgroup.ResultsCompare7、dwithControlgroup,theactivityofSODdecreased(P<0.05),thecontentofMDAincreased(尸<0.05),andthecontentofICAM一1increased(P<0.05)inLPSgroupandBregroup.ComparedwithLPSgroup,theactivityofSODincreased(P<0.05),thecontentofMDAdecreased(P<0.05)inlungtissues,andthecontentofIC8、AM一1decreased(Pl
3、低细胞间粘附分子一1的表达来减轻脂多糖致大鼠急性肺损伤。【关键词】急性肺损伤;灯盏花素;脂多糖;细胞间粘附分子一1【中图分类号】R-332【文献标识码】A【文章编号】1003-6350(2015)02~0163一o3Protectiveefectsofbreviscapineinjectiononacutelunginjuryinducedbyllpopolysaccharidesinrats.YANGQiu—di,LIJian-hui.DepartmentofInternalMedicine,HospitalofAnx
4、inCounty,Anxin071000,Hebei,CHINA【Abstract】ObjectiveToexploretheprotectiveeffectofbreviscapine(Bre)onacutelunginjuryinducedbylipopolysaccharides(LPS)inratsanditspossiblemechanisms.MethodsThirtymaleSprague-Dawleyratswereran—domlydividedintothreegroups:Controlgroup,
5、LPSgroup,Breviscapine(Bre)group,eachwith10rats.Controlgroupwasinjectedby0.1ml/kgnormalsaline.LPSgroupandBreviscapinegroupwasinjectedofLPSf5mg/kg).Ad—ditionally,Bregroupreceivedintraperitonealinjectionofbreviscapine(50mg/kg)30minutesbeforeLPSinjectionand30minafter
6、LPSinjection.Morphologicalchangesoflungtissueswereobservedunderlightmicroscope.Theac-tivityofsuperoxidedismutase(SOD),thecontentofmalondialdehyde(MDA)inlungtissuesandthecontentoflinter-cellularadhesionmolecule一1(CAM一1)inserumweredetectedineachgroup.ResultsCompare
7、dwithControlgroup,theactivityofSODdecreased(P<0.05),thecontentofMDAincreased(尸<0.05),andthecontentofICAM一1increased(P<0.05)inLPSgroupandBregroup.ComparedwithLPSgroup,theactivityofSODincreased(P<0.05),thecontentofMDAdecreased(P<0.05)inlungtissues,andthecontentofIC
8、AM一1decreased(Pl
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