2、50 g，无水磷酸 氢二钠0.724 g，磷酸二氢钾0.210g 溶于蒸馏水1000 mL 中，以1N 氢氧化钠 溶液调pH 值为7.0~7.4，经121℃灭菌15 分钟五、我准备用多聚赖氨酸涂片培养细胞,不知道多聚赖氨酸涂过的玻片如何灭菌？能否干烤灭菌？ 答：Sigma的产品说明书上写的很明白的。 另外，有本书上是这么写的，供参考：Poly-lysine-coated tissue culture surfaces To coat coverslips: Prepare a stock solution by disso
3、lving 25 mg/ml polylysine in 4.73 ml water (both poly-L-lysine and poly-D-lysine are used to coat tissue culture surfaces; check specific protocol for choice of isomer) and filter sterilize through a 0.22-μm filter. Store in 100-μl aliquots at ?20°C. When ready to use, dilute one al
4、iquot in 40 ml water to prepare 13 μg/ml working solution. Sterilize coverslips by autoclaving prior to coating. Dip coverslips in the working solution, then incubate 15 min to several hours in a humidified 37°C, 5% CO2 incubator. Allow surface to dry. To coat culture dishes o
5、r 8-well chamber slides: Prepare a stock solution by dissolving 100 mg poly-lysine in 100 ml water (both poly-L-lysine and poly-D-lysine are used to coat tissue culture surfaces; check specific protocol for choice of isomer) and filter sterilize through a 0.22-μm filter. Store in 5-
6、ml aliquots at ?20°C. When ready to use, dilute 1 part stock solution with 9 parts water to prepare 100 μg/ml working solution. Fill tissue culture dishes or slide wells with the working solution and incubate 1 hr in a humidified 37°C, 5% CO2 incubator, then remove solution by vacuu
7、m aspiration and allow surface to dry.Store coated tissue culture ware up to 3 months at 4°C. Use diluted solutions only once, but unused diluted aliquots can be stored up to 3 months at 4°C. 你可以配置好PLL后单独将其过滤除菌，分装，待用。玻片也是事先泡洗洁精，泡酸，双蒸水洗，烘干后高压灭菌，待用。培养的前一天包被培养板时先把玻片放入培养孔然后把PLL加到玻
