慢性重型乙型肝炎患者外周血DNT细胞及T细胞亚群的表达及意义-论文.pdf

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1、2248河北医药2014年8月第36卷第15期HebeiMedicalJournal，2014，Vol36AugNo．15doi：10．3969／j．issn．1002—7386．2014．15．002·论著·慢性重型乙型肝炎患者外周血DNT细胞及T细胞亚群的表达及意义刘坤杨亚萍刁青陈璀孙谢文【摘要】目的研究外周血CD3CD4-CDs-(DNT)细胞及T细胞亚群在慢性重型乙型肝炎患者的表达及意义。方法正常对照者32例、慢性乙型肝炎(CHB)40例、慢性重型乙型肝炎(CSHB)35例患者为研究对象，采用流式细胞仪检测各组外周血DNT细胞及T

2、细胞亚群。结果CHB组和CSHB组DNT细胞占T淋巴细胞的百分比与对照组比较明显增高(P<0．05)；而CSHB组增高程度更为显著，与CHB组比较差异亦有统计学意义(P<0．05)。HBVDNA载量与DNT细胞占T淋巴细胞的百分比呈正相关(r=0．742，P<0．05)。CHB组与对照组比较，仅co：亚群明显降低，差异有统计学意义(P

3、型肝炎发生发展的重要因素。【关键词】慢性重型乙型肝炎；DNT细胞；T细胞亚群【中图分类号】R512．62【文献标识码lA【文章编号】1002—7386(2014)15—2248—03ExpressionandsignificanceofdoublenegativeTcellsandlymphocytesubtypeinpatientswithchronicseverehepatitisBLIUKun，YANGYaping，DIAOQ，eta1．DepartmentofLiverDiseases，TheThirdHospitalofQinh

4、uangdaoCity，Hebei，Qinhuangdao066001，China【Abstract】ObjectiveToinvestigatetheexpressionandsignificanceofCD3+CD4-CDs-(DNT)cellsandlymphocytesubtypeinpatientswithchronicseverehepatitisB．MethodsTheserumsamplesfrom32healthysubjects，40patientswithchronichepatitis(CHB)and35patie

5、ntswithchronicseverehepatitisB(CSHB)werecollectedinthisstudy．TheDNTcellsandlymphocytesubtypeinperipheralbloodweredetectedbyflowcytometry．ResultsThepercentofDNTcellsinCHBandCSHBgroups[(8．44±4．25)％and(10．78±5．33)％，respectively]weresignificantlyhigherthanthoseincontrolgroup[

6、(6．23±3．77)％，P<0．05]，moreover，theincreaseextentinCSHBgroupwasmoreobviousthanthatinCHBgroup(P<0．05)．TheHBVDNAloadwaspositivelycorrelatedtoDNTcells(r=0．742，P<0．05)．TheCD；+TlymphocytesinCHBgroupweresignificantlydecreased，ascomparedwiththoseincontrolgroup(P<0．05)．Thepercentag

7、esofCD4TlymphocytesandtheratioofCD／CDsinCSHBgroupweresignificantlydecreased，however，CDs+Tlymphocytesweresignificantlyincreased，ascomparedwiththoseinCHBgroup(P<0．05)．ConclusionTheobviousincreaseofDNTcellsandtheabnormalityofTlymphocytesubsetpercentageinpatientswithchronicse

8、verehepatitisBmaybeoneofimportantreasonsforthepathogenesisanddevelopmentofchronichepatitisB．【Key