宫宁颗粒调控IUD模型大鼠子宫血管重塑机制的实验研究-论文.pdf

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1、·1208·世界中西医结合杂志2013年第8卷第12期WorldJournalofIntegratedTraditionalandWesternMedicine2013，Vo1．8，No．12·实验研究·宫宁颗粒调控IUD模型大鼠子宫血管重塑机制的实验研究岳恒学师伟刘瑞芬徐丽【摘要】目的探讨宫宁颗粒调控IUD(宫内节育器)模型大鼠子宫血管重塑机制。方法选定53只SD雌性大鼠，其中3只备用，余50只随机分为模型组、吲哚美辛组、宫宁颗粒组、假手术组、正常对照组。用免疫组化SP法(链霉菌抗生素蛋白过氧化物酶连结法)检测各组大鼠子宫血管平滑肌细胞(VSMC)表型标志

2、物a—SM一肌动蛋白(a—SM—action)和VSMC增殖细胞核抗原(PCNA)的表达，计算子宫VSMC增殖率(PCNA／a—SM—action阳性细胞数x100％)。结果宫宁颗粒组a—SM—action(％)为45．70±6．20、PCNA为41．32±9．28、PCNA／a—SM—action为0．93±0．26，均接近正常对照组。结论宫宁颗粒是通过调控a—SM—action和PCNA的表达，促使VSMC由病理性合成表型VSMC逆转为生理性收缩表型VSMC，从而使IUD模型大鼠受损的子宫内膜血管由病理性重塑逆转为适应性重塑，进而恢复子宫血管正常生理功能

3、。【关键词】宫宁颗粒；调控；IUD模型大鼠子宫血管重塑机制；实验研究【中图分类号】R285．5；R713．9【文献标识码】A【文章编号】1673—6613(2013)12—1208—04ExperimentalStudyoftheMechanismofGongningGranulesontheRegu-lationofUterineVascularRemodelinginIUDModelRatsYUEHeng—xue，SHIWei，LIURui一n，XULi(1．2010DoctorateCandidateofShandongUniversityofTrad

4、itionalChineseMedicine．JinanShandong250011；2．AffiliatedHospitalofShandongUniversityofTraditionalChineseMedicine．JinanShandong250011；3．ProvincialHospitalofShandongUniversity．JinanShandong250211)【Abstract】ObjectiveTodiscussthemechanismofGongningGranulesontheregulationofuterinevascula

5、rremodelinginIUD(intrauterinedevice)modelrats．MethodsFifty—threeSDfemaleratswerese—lected．Threeofthemwerebackupandtherest50ratswererandomizedintoamodelgroupanIndometacingroup，aGongningGranulesgroup，apseudo—operationgroupandanormalcontrolgroup．ImmunohistoehemiealSP(steptavidin—peros

6、idase)methodwasusedtodetecttheexpressionsofa—SM—actionandPCNA(proliferaringcellnuclearantigen)inuterineVAMC(vascularsmoothmusclecells)phenotypicmarker．VSMCprolifera—tingrate(PCNA／a—SM—actionpositivecellcount×100％1wascalculated．ResultsTheexpressionofa—SM—actionwas(45．70±6．20)，thatof

7、PCNAwas(41．32±9．28)andPCNA／a—SM—actionwas(0．93±0．26)inGongningGranulesgroup．whichwasneartotheresultsinthenormalcontrolgroup．ConclusionGongningGranulespromotethereversionofpathologicalpathologicsyntheticphenotypeVSMCintophysiologi—calcontractionphenotypeVSMCviatheregulationofa——SM—．

8、actionandPCNAexpressionsso