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《三疣梭子蟹软壳蟹肝胰腺的消化酶活力与营养成分分析-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第33卷第2期生态科学33(2):237-2432014年3月EcologicalScienceMar.2014靳立兵,王春琳,母昌考,等.三疣梭子蟹软壳蟹肝胰腺的消化酶活力与营养成分分析[J].生态科学,2014,33(2):237—243.JINLibing,WANGChunlin,MUChangkao,eta1.AnanalysisofthedigestiveenzymesactivitiesandnutritivecompositioninthehepatopancreasofsoftshellPortunustrituberculatus[J].EcologicalScience
2、,2014,33(2):237—243.三疣梭子蟹软壳蟹肝胰腺的消化酶活力与营养成分分析靳立兵,王春琳,母昌考,宋微微,李荣华宁波大学海洋学院,宁波315211【摘要】通过生化分析、气质联用和液质联用等技术,系统地研究了三疣梭子蟹(Portunustrituberculatus)软壳蟹肝胰腺的消化酶活力与营养组成。结果表明三疣梭子蟹软壳蟹肝胰腺中粗脂肪的含量最高,达47.97%,但脂肪酶活力却最小;其消化酶活力大小为:淀粉酶>类胰蛋白酶>脂肪酶,且各消化酶活力之间差异显著(尸l3、PUFA)12种;其中SFA的含量最高,为33.05%,PUFA次之,占32.45%,MUFA的含量最少,为28.18%。氨基酸组成中谷氨酸含量居首位,共检测出29种氨基酸,包括7种必需氨基酸、2种半必需氨基酸及2O中非必需氨基酸;非必需氨基酸占总氨基酸的比例最高,为52.77%;其次为必需氨基酸,占35.39%:半必需氨基酸最低,为11.84%。由此可知,脂类物质是三疣梭子蟹软壳蟹期间主要的能源物质,其脂肪酸和氨基酸的种类及含量与硬壳蟹相比存在着一定的差异。关键词:软壳蟹;肝胰腺;消化酶;常规营养成分;脂肪酸;氨基酸doi:10.3969~.issn.1008.8873.2014.02.4、006~1It@类号:$912文献标识码:A文章编号:1008—8873(2014)02-237—07Ananalysisofthedigestiveenzymesactivitiesandnutritivecompositioninthehepat0pancreasofsoftshellPortunustrituberculatusJINLibing,WANGChunlin’,MUChangkao,SONGWeiwei,LIRonghuaMarineCollegeofNingboUniversity,Ningbo315211,ChinaAbstract:Itstudiedthehepat5、opancreasdigestiveenzymesandnutritionalcompositionofsoftshellportunustrituberculalusbybiochemicalanalysis.GC.MSandLC.MStechnologies.TheresultsshowedthatthecontentofcrudefatinhepatopancreasofsoftshellProtunustrituberculatuswasthehighestuDtO47.97%,butthespecificactivitiesoflipasewerelowest;thedigest6、iveenzymesactivitiesweresignificantlydiferentinhepatopancreas<0.05)respectively,amylase>tryptase>lipase.Atotalof38kindsoffattyacidsincludedl8kindsofsaturatedfattyacids(SFA),8kindsofmonounsaturatedfattyacids(MUFA),12kindsofpolyunsaturatedfattyacids(PUFA1:thecontents0fSFA,MUFAandPUFAwere33.05%,28.187、%and32.45%.SFAwasthehighest.Theglutamicacidcontentwasthehighestinaminoacids.29kindsofaminoacidswerepresentinthehepatopancreasofsoftshellProtunustrituberculatus,c0getherwiththecontentof7essenfia1aminoacids,2semi—e
3、PUFA)12种;其中SFA的含量最高,为33.05%,PUFA次之,占32.45%,MUFA的含量最少,为28.18%。氨基酸组成中谷氨酸含量居首位,共检测出29种氨基酸,包括7种必需氨基酸、2种半必需氨基酸及2O中非必需氨基酸;非必需氨基酸占总氨基酸的比例最高,为52.77%;其次为必需氨基酸,占35.39%:半必需氨基酸最低,为11.84%。由此可知,脂类物质是三疣梭子蟹软壳蟹期间主要的能源物质,其脂肪酸和氨基酸的种类及含量与硬壳蟹相比存在着一定的差异。关键词:软壳蟹;肝胰腺;消化酶;常规营养成分;脂肪酸;氨基酸doi:10.3969~.issn.1008.8873.2014.02.
4、006~1It@类号:$912文献标识码:A文章编号:1008—8873(2014)02-237—07Ananalysisofthedigestiveenzymesactivitiesandnutritivecompositioninthehepat0pancreasofsoftshellPortunustrituberculatusJINLibing,WANGChunlin’,MUChangkao,SONGWeiwei,LIRonghuaMarineCollegeofNingboUniversity,Ningbo315211,ChinaAbstract:Itstudiedthehepat
5、opancreasdigestiveenzymesandnutritionalcompositionofsoftshellportunustrituberculalusbybiochemicalanalysis.GC.MSandLC.MStechnologies.TheresultsshowedthatthecontentofcrudefatinhepatopancreasofsoftshellProtunustrituberculatuswasthehighestuDtO47.97%,butthespecificactivitiesoflipasewerelowest;thedigest
6、iveenzymesactivitiesweresignificantlydiferentinhepatopancreas<0.05)respectively,amylase>tryptase>lipase.Atotalof38kindsoffattyacidsincludedl8kindsofsaturatedfattyacids(SFA),8kindsofmonounsaturatedfattyacids(MUFA),12kindsofpolyunsaturatedfattyacids(PUFA1:thecontents0fSFA,MUFAandPUFAwere33.05%,28.18
7、%and32.45%.SFAwasthehighest.Theglutamicacidcontentwasthehighestinaminoacids.29kindsofaminoacidswerepresentinthehepatopancreasofsoftshellProtunustrituberculatus,c0getherwiththecontentof7essenfia1aminoacids,2semi—e
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