TRAIL联合水飞蓟素对人肝癌Huh7细胞凋亡的影响-论文.pdf

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1、·562·免疫学杂志2014年6月第3O卷第6期IMMUNOLOGICALJOURNALVo1．30No．6Jun．2014·短篇·~-]1o0o一8861(2014)06—0562—04；[DO1]IO．134316．cnki．immuno1．j．20140123TRAIL联合水飞蓟素对人肝癌Huh7细胞凋亡的影响徐志辉，赵金金，尤红娟，胡磊，罗文雅，李向阳，郑葵阳，陈明，汤仁仙【摘要】目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合水飞蓟素(Sili)对人肝癌Huh7细胞凋亡的影响。方法CCK8法检测细胞增殖抑制：分光光度法检测细胞caspase一

2、3、caspase一8、caspase一9相对活性；Westernblot检测细胞表面死亡受体4(DR4)、死亡受体5(DR5)的表达。结果Sili与rrRAIL联合作用Huh7细胞后，联合用药组与对照组及单独用药组相比，Huh7细胞的增殖率明显下降；caspase～3、caspase一8、caspase一9的相对活性明显提高。Sili作用Huh7细胞后能明显提高DR4、DR5蛋白表达。结论Sili可通过上调死亡受体DR4、DR5表达而促进TRAIL诱导的Huh7细胞凋亡，为肝细胞癌的临床治疗提供了新的思路和方法。[关键词】TRAIL；水飞蓟素；肝细胞癌；凋

3、亡【中图分类号1R730．5l【文献标识码1BTheefectsofTRAILcombiningSilibininonapoptosisofhumanhepatomacarcinomacelllineHuh7XUZhihui'2ZHAOJinjin，YOUHongjuan，HULei，LUOWenya，LIXiangyang，ZHENGKuiyang，CHENMing2,TANGRenxian，．DepartmentofPathogenicBiologyandImmunologyLaboratoryofInfectionandImmunity,XuzhouM

4、edicalCollege，Xuzhou221004,China,"2．DepartmentofInfectionsDisease，AffiliatedHospitalofXuzhouMedicalCollege，Xuzhou221002，ChinaCorrespondingAuthor：TANGRenxian,E-mail：tangrenxian-t@163．corn[Abstract]Tumornecrosisfactor—relatedapoptosis—inducingligand(TRAIL)isapromisinganti—canceragent

5、whichselectivelyinducesapoptosisincancercells．However，resistancetoTRAIL-inducedapoptosisisanimportantandfrequentproblemincancertreatment．ThepresentstudyinvestigatestheeffectsandmechanismsofSilicombinationwithTRAILonhumanhepatomacarcinomacellline(Huh7cells)，whichareresistanttoTRAIL—

6、inducedapoptosis．CCK8showedthatSiliandTRAILsynergisticallyinducedcelldeathinHuh7cellline；eolorimetricassaysillustratedthatSiliandTRAILpromotedsynergisticactivationofcaspase-3，-8，and-9．Furthermore，WesternblotdemonstratedthatSilicouldup-regulatedeathreceptor4(DR4)andDR5expression．The

7、sefindingsdemonstrateSiliandTRAILcansynergisticallypromoteHuh7cellapoptosis，suggestingchemopreventiVeandtherapeuticpotentia1．[Keywords]RAIL；Silibinin；Hepatocellularcarcinoma；Apoptosis肝细胞癌(hepatocellularcarcinomaHCC)是导正常细胞几乎没有细胞毒性而被认为是一个很有潜致人类死亡的第2大全身性肿瘤疾病，且发病率逐力的抗癌药回。然而，在包括肝癌细胞在内的许

8、多肿瘤年上升，全世界每年大约有750000新发肝癌病