基于肝毒性的雷公藤复方配伍减毒作用及机制研究-论文.pdf

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1、106中药药理与临床2013；29(5)cells．Methods：50micewel'edividedinto5groups，nanlelythenormalsalinegroup(controlgroup)，FuzhengYiliuDecoctionhigh，medi—umandlowdosegroup，5-Fugroup，10ratsineachgroup，establishmentofhumanhepatocellularcarcinomaHepG-2nudemousemodel，the,rumorvolumebegantousegrowstoabout100nun

2、3，FuzbengYiliudecoctionintragastricadministration(oncead叼)，group5-Fu(twiceaweek)andphysiologicalsalinecontrolgroupbyperitonealinjection(onceaday)，administeredfor18days，tostoptheverynextdayeachgroupwereeuthanized，strippingthetumorweighi“g，tumorgrowthcurvewasdrawn，calculationthetumorinhibiti

3、onrate，fromtumortissue，TunelstainingwasusedtodetecttheefectofFuzhengYiliuDecoctionontheapeptosisoftumorcells；transmissionelectronmicroscopy(TEM)detectionofautophagosomeformation，Westernblotdetectingofautophagy—relatedprotein(Beclin1，Bnip3andLC3)expressionchanges．Results：FuzhengYiliuDecocti

4、oninhigh，me~umandlowdosegroup，thetumorinhibitionratewere49．9％，21．6％，8．2％，group5-Fuinhibitoryratewas74．2％，comparedwiththecontrolgroup，FuzhengYliuDecoctionhighdosegroup(32g／kg)，middledosegroup(16g／ks)and5-Fugroupcouldinhibitthegrowthoftumor，thedosegroupandthe5-Fugrouphasthebestefect，thedifer

5、enceofFuzhengYiliuDecoctionlowdosegroupwa$notobvious(P>0．01)；Tunelstainingshowedthat，compamdwiththecontrolgroup，druggroupand5-Fugroupcouldseealotofapoptosisoftumorcels，whichinhi#dosegroupofinducingtumorcellsapoptosisefectbest，FuzhengYiliudecoctionmiddledosegroup，lowdoseandthecontrolgroupha

6、dnosignificantdiference；electronmicroscoperesultsshow：druggroupand5-Fugroupcouldseealotofautophagosome．withdrugconcentrationdecreasedautophagicvacuoledecreased，andtherewasapositivecorrelationbetweenthem，thehighdosegroupwasthebest，thecontrolgroupwasnotfoundtheautophagosome；Westen—blottestre

7、sultsshowthat：comparedwiththecontrolgroup，druggroupand5-Fugroupscanmakeautophagy-relatedproteinBeclin1，Bnip3andLC3expression，andtheconcentrationofthedrugincorrelation，theefectofhighdosegroupwasthebest．Condusion：FuzhengYiliudecoctioncaninhibitthegrowthoftumorce