PIWIL2蛋白在肝门部胆管癌组织和细胞株中的表达及临床意义-论文.pdf

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1、·280·中华肝胆外科杂志2014年4月第2O卷第4期ChinJHepatobiliarySurg，April2014，V01．20，No．4·论著·PIWIL2蛋白在肝门部胆管癌组织和细胞株中的表达及临床意义温松奇熊小凡杨光耀杨康俞亚红丁志强游建陈勇军【摘要】目的研究PIWI12在肝门部胆管癌组织和细胞株中的表达及其与临床病理特征和根治性切除术预后的关系。方法采用免疫组织化学方法检测41例肝门部胆管癌和10例正常胆管组织中PIWI12的表达；蛋白印迹和细胞免疫荧光技术检测人胆管癌细胞系QBC。。和人正常胆管上皮细胞HIBEpic株中PIWIL2的表达。采用Kaplan—Mei

2、er法对胆管癌根治术后患者进行生存期的单因素及多因素分析。结果PIWIL2在肝门部胆管癌和QBC。。细胞中的表达明显高于对照组织和HIBEpic细胞(P<0．05)；PIWI12在低、中分化胆管腺癌的表达明显高于高分化腺癌(P<0．05)。单因素生存分析显示根治术后PIWIL2高表达的生存期较短(P<0．05)。多因素分析显示PIWI12的表达水平是影响胆管癌根治术预后的独立危险因素(P<0．05)。结论PIWIL2在肝门部胆管癌组织和细胞系中表达上调，其表达水平与肿瘤分期及分化程度相关，是影响肝门部胆管癌根治切除预后的独立危险因素。【关键词】PIWI蛋白，PIWIL2；肝门部

3、胆管癌；预后；危险因素ExpressionandclinicalsignificanceofPIWIL2inhilarcholangiocarcinomatissuesandincelllinesnSongqi，XiongXiaofan，Guangyao，Kang，Yahong，DingZhiqiang，YouJian，ChenYongjun．DepartmentofHepatobiliaryPancreaticandHerniaSurgery，AffiliatedPuaiHospital，TongMedi—calCollege，HuazhongUniversityofScie

4、nceandTechnology，Wuhan430000，ChinaCorrespondingauthor：ChenYongjun，Email：chenyongjun45@126．corn【Abstract】ObjectiveToexploretherelationshipbetweenP1wII2andclinic叩athologicalcharac—teristiesandprognosisafterradicalresectionbydetectingtheexpressionofPIWII2inhilarcholangiocarcino．mstissuesandince

5、lllines．MethodsTheexpressionofPIWI【2wasdetectedbyimmunohistochemistryin41hilarcholangiocarcinomasand10controltissues，Western．blotandimmunocytofluorescentwereusedtoinvestigatethePIWII2expressionincholangiocarcinomacelllinesQBC939andbileductepithelialcelllinesHIBEpic．Univariateandmultivariatea

6、nalysisofsurvivalwasperformedusingtheKaplan—Meiercurveinhi—larcholan~ocarcinomapatientswhohadunderwentradicalresection．ResultTheexpressionofPIWI12inhilarcholangiocareinomatissuesandQBC939cellsweresignificantlyhigherthanthecontroltissuesandHIBEpiccells(P<0．05)．TheexpressionofPIWI12inpoorandmo

7、deratediferentiatedcholangiocarcino—mawassignificantlyhigherthanthewelldifferentiatedgroup(P<0．05)．UnivariateanalysisshowedthathighexpressionofPIWIL2wasassociatedwithshort—survivaltimeafterradicalresection(P<0．05)．Muhi—variateanalysisshowedthatthee