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页数:8页
时间:2020-05-03
《基于耐药特性联合单细胞培养识别肝癌干细胞克隆亚群-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
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3、l-盎@世界华人消化杂志2014~1月28日;22(3):319—326wcjd@wjgnet.cornISSN1009—3079(print)ISSN2219—2859(online)罐蟛《蕊基于耐药特性联合单细胞培养识别肝癌干细胞克隆亚群陈娟,区泳芳,蔡捷,陶璐,陈相宜,邝晓聪陈娟,区泳芳,蔡捷,陶璐,邝晓聪,广西医科大学病理与病edsubcutaneouslyinnudemicetoinducetumor一背景资料理生理教研室广西壮族自治区南宁市530021陈相宜,广西医科大学
4、生理教研室广西壮族自治区南宁市formati。andamycin(8mg/kg)interven一纂耋茬‘孬蠢量530021tionwasgiven.Whenthetumordiameterwas二.陈娟,硕士,主要从事癌干细胞的研究.1.5cm,tumortissueswerecollectedforprimary全克隆细胞群富广西自然科学基金资助项目,Nos.桂科自0991139,2013GXculture.Thecellswereinoculatedsubcutane.含癌干细胞,从肝NSFAA
5、O19244广西科学研究与技术开发计划基金资助项目,No.桂科攻()9。uslyinnudemceagain.terfourc。nsecutVe辈皇袭930()3C一1generationsinnudemice,thetumorcellswere矗重:作者贡献分布:本文由陈娟撰写邝晓聪负责实验没计区泳named”BEL一7404一ADM—P4”.Basedonthetu.芳、蔡捷、陶璐及陈相宜负责实验数据的收集和整理morformingtime,theenrichmentoflivercancer通讯
6、作者:邝晓聪副教授,硕士生导师,530021,广西南宁市双拥路22号.厂西医科大学病理与病理生理教研室.stemcellswastesfed.Usingunicellularculture,1609800049@qqcomclonesfromBEL..7404andBEL.7404..ADM..P4电话:0771—5358502cellsweredividedintoholoclones,meroclones收稿日期:2013~09—30修回日期:2013—11—21接受日期:2013~12一O5在线
7、出版日期:2014—01—28andpareclonestotestthecancerstemcellchar.acteristicsfrenewa1ability,cloneformationandsphereformation1.Holoclones,meroclonesandIdentificationoflivercancerparecloneswerestainedwithhoechst33342andstemce¨s-containingcellobservedunderaconfocal
8、microscope.Basedsubgroupsbasedonresistanceontherenewa1ability,cloneformationrateandsphereformationrateandhoechst33342stain—characteristicsandunicellularingproperties,cancerstemcellssubpopulationscultureJuanChen,Yong—FangOu,JieCai,LuTao,Xiang-YiChen,Xiao
9、。CongKuangRESUL.TS:Thetumorformationratewas100%,andtumorformationtimewasshortenedfromthefirstgenerationtothefourthgeneration,JuanChen,Yong·FangOu,JieCai,LuTao,Xiao-CongKuang,DepartmentofPathologyandPathophysiology,whichsuggestedthepreliminaryenrichmento
10、fGuangxiMedicalUniversity.Nanning530021.Guangxilivercancerstemcells.HoloclonesshowedtheZhuangAutonomousRegion。Chinafastestgrowthandthelargestcellvolume,fo1.Xiang-yiChen,DepartmentofPhysiology,GuangxiMedi—calUniversity,Nanning5300
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