T单核苷酸多态性与代谢综合征的相关性.pdf

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1、宁夏医学杂志2014年9月第36卷第9期NingxiaMedJ，Sep．2014，Vol36，No．9·781·Doi：10．13621／j．1001—5949．2014．09．0781著·宁夏回汉族人群脂联素基因SNP+276G／T单核苷酸多态性与代谢综合征的相关性武海亮‘，沈佳，陈芳。，刘雅娟，杨锐英[摘要]目的探讨宁夏回、汉族人群脂联素基因SNP+276G／T单核苷酸多态性与代谢综合征(MS)的相关性。方法收集回、汉族MS患者(MS组)207例和136例以及回、汉族健康体检者(对照组)98例和102例，采用PCR—

2、RFLP技术检测SNP+276G／T单核苷酸多态性，用ELISA法检测血清脂联素的浓度。结果汉族MS组GG基因型频率及G等位基因频率均高于汉族对照组(P<0．05)，回汉族2组间基因型频率及等位基因频率差异均无统计学意义(P>0．05)。回、汉族MS组血清脂联素水平均低于其对照组(P<0．05)，回、汉族TG+1TI’基因型血清脂联素水平低于GG基因型者。Logistic回归分析显示SNP+276基因多态性是汉族MS的危险因素。结论脂联素基因SNP+276G／T多态性可能与宁夏汉族MS相关，与宁夏回族MS无相关性。[关键

3、词]代谢综合征；脂联素；基因多态性；宁夏[中图分类号】R394[文献标识码】ARelationshipsbetweenadiponectingene+276nucleotideT／GpolymorphismwithmetabolicsyndromeinHuiandHannationalitiesinNingxiaWUHailiang，SHEN，CHENFang，LIUYajuan。，YANGRuiying．1．DepartmentofGerontology，GeneralHospitalofNingxiaMedicalU

4、niversity，Yinchuan750004，China；2．ICU，CardiovascularandCerebrovascularDiseasesHospitalofGeneralHo~italofNingxiaMedicalUniversity，Yinchuan750002，China；3．DepartmentofCardiology，YinchuanFirstPeople~Hospital，Yinchuan750001，ChinaCorrespondingauthor：YANGRuiying，Email：an

5、gelwhl@163．com[Abstract]ObjectiveToinvestigatetherelationshipbetweenadiponectin(APN)gene+276nueleotideG／Tpolymorphismwithmetabolicsyndrome(MS)inHuiandHannationalitiesinNingxia．MethodsThestudywasperformedin207Huipatientsand136HanpatientswithMS(MSgroup)andwasperfor

6、medin98Huihealthypeopleand102Harthealthypeople(controlgroup)．PCR—RFLPandELISAwereusedtodetectthegenepolymorphismofAPNandtheserumAPNleve1．ResultsThefrequenciesofGGgeno—typeandGalleleinHanMSgroupwerehigherthanthoseinHancontrolgroup(P<0．05)．Thefrequenciesofgenotypea

7、ndallelewerenotsignificantlydifferentbetweenHuiMSgroupandHuicontrolgroup(P>0．05)．Comparedwiththecontmlgroup，theserumAPNlevelinMSgroupwaslowerinbothHuiandHannationalities(P<0。05)．TheserumAPNlevelinsubjectwithTG+TTgenotypeswasandHannationality(P<0．05)．Logisticregre

8、ssionanalysisshowedthatAPN+276G／TgenepolymorphismsweretheriskfactorsformetabolicsyndromeinHansubjects．Conclusion+276G／TpolymorphismofAPNmightbeassociatedwithMS