资源描述：
《渗透压敏感和耐高渗酵母菌在海藻酸钠-壳聚糖-海藻酸钠微囊中的生长和代谢》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、生物工程学报ChinJBiotech2008,July25;24(7):1274-1278journals.im.ac.cnChineseJournalofBiotechnologyISSN1000-3061cjb@im.ac.cn©2008InstituteofMicrobiology,CAS&CSM,Allrightsreserved研究简报渗透压敏感和耐高渗酵母菌在海藻酸钠-壳聚糖-海藻酸钠微囊中的生长和代谢1,212111111宁小娟,孙志杰,钟盛华,林军章,吕国军,于炜婷,朱静,王为,马小军1中国科学院大连化学物理研究所生物医
2、学材料工程组,大连1160232江西农业大学理学院,南昌330045摘要:为了研究微囊微环境中渗透压对微囊内不同渗透压敏感性细胞生长、代谢的影响,分别以渗透压敏感型酿酒酵母Y02724与耐高渗酵母Hansel为细胞模型,考察了有氧条件下这两种细胞在海藻酸钠-壳聚糖-海藻酸(Alginate-chitosan-alginate,ACA)微胶囊中的生长、代谢状态。主要检测了细胞比生长速率、最大产物生成量以及代谢物乙醇、甘油分泌量等的变化。实验结果分析表明,渗透压胁迫可能是导致不同渗透压敏感性细胞在微囊微环境中生长代谢特征变化的因素之一,即微
3、囊微环境内可能存在渗透压胁迫。关键词:酵母,渗透压胁迫,ACA微囊,微囊微环境,生长代谢GrowthandMetabolismofOsmo-sensentiveYeastY02724andHigh-osmoticResistantYeastHanselinAlginate-chitosan-alginateMicrocapsules1,212111XiaojuanNing,ZhijieSun,ShenghuaZhong,JunzhangLin,GuojunLü,WeitingYu,111JingZhu,WeiWang,andXiaoju
4、nMa1DalianInstituteofChemicalPhysics,ChineseAcademyofSciences,Dalian116023,China2CollegeofScience,JiangxiAgriculturalUniversity,Nanchang330045,ChinaAbstract:Tostudytheeffectoftheosmoticstressinthemicroenvironmentonthegrowthandmetabolismoftheencapsulatedcellsunderaerobicc
5、ondition,Osmo-sensitiveyeastY02724andhigh-osmoticresistantyeastHanselwereusedasmodelstoexplorethegrowthandmetabolismstateofthecellscultivatedinalginate-chitosan-alginate(ACA)microcapsules.Thechangesoftheyeastcells’specificgrowthrate,maximumproductquantityandthesecretiono
6、fethanolandglycerolwereanalyzed.ForY02724,theyieldofethanolwasincreasedintheACAmicroenvironmentcomparedtosuspensioncultivation.ForHansel,themaximumgrowthspeedofmicroencapsulatedcultivationhadnoobviousdifferencecomparedtothesuspensioncultivation.Moreover,afterencapsulatio
7、n,theproductionofglycerolwasdecreasedforbothY02724andHanselcomparedtosuspensioncultivation.Inconclusion,osmoticstressexistedintheACAmicrocapsulesandaffectedthegrowthandmetabolismofthecells.Keywords:yeast,osmoticstress,alginate-chitosan-alginatemicrocapsule,microenvironme
8、nt,growthandmetabolismReceived:October29,2007;Accepted:January16,2008Supportedby:theNationalNaturalScie
