腹泻患者肠道菌群数量变化及ERIC-PCR指纹图谱分析.pdf

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1、中国医药导报2014年11月第11卷第32期窑论著窑腹泻患者肠道菌群数量变化及ERIC-PCR指纹图谱分析12银11刘海燕曹海涛常玉梅徐鹏1.解放军第252医院中心实验室袁河北保定071000曰2.解放军第252医院医务处袁河北保定071000[摘要]目的了解粪检有白细胞的腹泻患者与健康者肠道菌群变化及指纹图谱差异遥方法提取解放军第252医院临床门诊30名健康成人和30例腹泻成人的粪便总DNA为模板袁分为对照组和腹泻组遥荧光定量法测定肠道内双歧杆菌尧乳酸杆菌尧大肠埃希菌尧肠球菌的菌群数量袁ERIC-PCR指纹图谱法检查各组肠道菌群组成多

2、样性差异遥结果两组肠道菌群数量院对照组粪便中双歧杆菌为渊8.85依0.57冤拷贝/g袁乳酸杆菌为渊8.36依0.45冤拷贝/g曰腹泻组粪便中双歧杆菌为渊8.28依0.68冤拷贝/g袁乳酸杆菌为渊7.79依0.39冤拷贝/g曰与对照组比较袁腹泻组中该两种细菌数量明显减少渊P<0.05冤遥大肠埃希菌和肠球菌数量在两组之间差异无统计学意义渊P>0.05冤遥腹泻患者肠道菌群ERIC-PCR指纹图谱条带明显减少遥结论腹泻患者的肠道菌群结构发生了改变袁菌群数量明显减少遥[关键词]腹泻曰荧光定量PCR曰ERIC-PCR曰肠道菌群[中图分类号]Q78[

3、文献标识码]A[文章编号]1673-7210渊2014冤11渊b冤-0021-05Analysisofthechangesofintestinalmicrofloraindiarrheapatientsbyfluorescentquantitativereal-timePCRandERIC-PCR12银11LIUHaiyanCAOHaitaoCHANGYumeiXUPeng1.CentralLaboratory,No.252HospitalofPLA,HebeiProvince,Baoding071000,China;2.Departm

4、entofMedicine,No.252HospitalofPLA,HebeiProvince,Baoding071000,China[Abstract]ObjectiveToanalyzethediversityvariationandthefingerprintsdifferenceofintestinalmicroflorabetweendiarrheapatientswiththefecalleukocytesandhealthpeople.Methods30casesofnormalpeopleand30casesofdi鄄a

5、rrheapatientsinNo.252HospitalofPLAwereselected,whosetotalfecalDNAwereusedasmodel,weredividedintocontrolgroupanddiarrheagroup.IntestinalBifidobacterium,Lactobacillus,EscherichiaColi,Enterococcibacterianumberweredetectedbyquantitativefluorescencemethod,thedifferenceofintes

6、tinalfloradiversityinthetwogroupswasdetectedbyERIC-PCRfingerprintmethod.ResultsThequantitiesofintestinalmicroflorainthecontrolgroupanddi鄄arrheagroup:Bifidobacteriumspp.[(8.85依0.57)copies/gvs(8.28依0.68)copies/g];Lactobacillusspp.[(8.36依0.45)copies/gvs(7.79依0.39)copies/g],

7、thereweresignificantdifferences(P<0.05).TherewasnodifferenceabouttheEscherichiaColiandEnterococciinthetwogroups(P>0.05).ThebandsofERIC-PCRfingerprintsindiarrheagroupwereobviouslydeclined.ConclusionTheconstitutionoftheintestinalmicrofloraischangedindiarrheapatientsandtheq

8、uantityofintestinalfloraissignificantdeclined.[Keywords]Diarrhea;Fluorescentquantificationreal-timePCR;