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时间:2020-04-27
《GDF15基因在卵巢上皮性癌组织中的表达及其临床意义-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、现代生物医学进展wwwa~hengwuyixue.comProgressinModernBiomedicineVoL14NO~29OCT~2014·5665·doi:10.13241~.cnki.pmb.2014.29.017·I1~2g,究·GDF15基因在卵巢上皮性癌组织中的表达及其临床意义术李博张颖。辛晓燕(1第四军医大学西京医院妇产科陕西西安710032;2解放军第323医院妇产科陕西西安710054)摘要目的:探讨生长分化因子GDF15(GrowthDiferentiationFactor15)基因在卵巢上皮性癌组织中的表达及其与铂类耐药的相关性。方法:应用免疫组化、westernb
2、lot、RTPCR等方法对80例原发性卵巢癌组织和卵巢癌顺铂敏感/耐药株A2780和CP70、SKOV3和SKOV3/DDP中生长分化因子GDF15表达水平进行测定。结果:生长分化因子GDF15的表达强度与卵巢癌铂类耐药性显著相关。在卵巢癌顺铂耐药株CP70、SKOV3/DDP中GDF15表迭水平较顺铂敏感株A2780、SKOV3明显增高。结论:GDF15表达水平与卵巢癌发生发展及铂类耐药相关,对于卵巢癌患者早期筛选、预测预后具有一定的临床指导价值。关键词:卵巢肿瘤;生长分化因子15;顺铂;耐药中图分类号:R737_31文献标识码:A文章编号:1673—6273(2014)29—5665—06
3、TheClinicalSignificanceOfGDF15ExpressioninHumanOvarianCancer木LIBo1,ZttANGYing‘,XINXiao-yan越(1DepartmentofObstetricsandGynecology,TheForthmilitaryMedicalUniversity,Xi'anShaanxi.710032,China;2DepartmentofObstetricsandGynecology,323militaryHospital,Xi'an,Shaanxi,710054,China)ABSTRACTObjective:Tostudyth
4、erelationshipoftheGrowthDiferentiationFactor15(GDFI5)expressioninhumanovariancancerandtheresistantofhumanepithelialovariancancertocisplatin.Methods:ExpressionandlocationofGDF15proteinwasdetectedinspecimensof80humanepithelialovariancancerpatientsand4humanovariancancercelllines.Results:cytoplasmicloca
5、lizationofGDF15positivestainingcouldbeobservedinhumanepithelialovariancancer.TheintensityofGDF15proteinwassignificantlycorrelatedwithFIGO,diferentiation,malignantascites,lymphaticmetastasis,andplatinumresistanceinovariancancer.GDF15mRNAlevelsincisplatinresistantovariancancercelllinesCP70andSKOV3/DDP
6、increasedsignificantlycomparedtothoseincisplatinsensitivecelllinesA2780andSKOV3:Treatedwithcisplatin.GDF15proteinlevelswereup.regulatedinbothA2780andCP70cells.Conclusion:GDF15expressionappeartodiferbetweenplatinumsensitiveandplatinumresistantovariancancers.ThusGDF15expressionmaybeusefulbiomarkerfore
7、arlyscreeningandpredictingprognosisinovariancancerpatients.Keywords:Ovariancancer;GDF15gene;Cisplatin;Chemo—resistanceChineseLibraryClassification:R737.31Documentcode:AArticleID:1673.6273(2014)29-.566
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