核黄素对接种tylcv后番茄幼苗生长和活性氧代谢的影响

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1、上海农业学报2013，29(6)：59—63ActaAgriculturaeShanghai文章编号：1000．3924(2013)06—059．05核黄素对接种TY[~V后番茄幼苗生长和活性氧代谢的影响黑银秀，刘君，郭世荣，朱为民(浙江省台州市农业科学研究院园艺所，台州317000；南京农业大学园艺学院，农业部南方蔬菜遗传改良重点开放实验室，南京210095；上海市农业科学院园艺研究所，上海市设施园艺技术重点实验室，上海201403)摘要：以番茄品种‘1479’为材料，研究了喷施2．0mmol／L核黄素对接

2、种番茄黄化曲叶病毒(TYLCV)后番茄幼苗生长、活性氧代谢的影响。结果表明：接种TYLCV显著抑制了番茄幼苗的生长；番茄幼苗叶片内MDA含量、0：·一产生速率和H0含量上升，CAT、APx活性呈上升趋势，而SOD活性降低；核黄素预处理能有效缓解TYLcV对番茄幼苗生长的抑制，显著降低了接种TYLCV后番茄幼苗体内MDA、HO含量和O：·一产生速率，提高了SOD、CAT、APX活性。说明2．0mmol／L核黄素可减少接种后番茄叶片内活性氧的产生，提高抗氧化酶的活性、降低膜脂过氧化水平，减缓番茄黄化曲叶病毒对植株

3、的伤害，增强番茄幼苗对TYLcV的抗性。关键词：番茄；核黄素；番茄黄化曲叶病毒；幼苗生长；活性氧代谢中图分类号：$436．412．1文献标识码：AEffectsofriboflavinongrowthandactiveoxygenmetabolismoftomatoseedlingsinoculatedwithtomatoyellowleafcurlvirusHEIYin-xiu’，LIUJun，GUOShi—rong，ZHUWei-min(HorticulturalResearchInstitute，Tai

4、zhouAcademyofAgriculturalSciences，Taizhou317000，China；CollegeofHorticulture，NanjingAgriculturalUniversity；KeyLaboratoryofSouthernVegetableCropGeneticImprovement，MinistryofAgriculture，Nanjing210095，China；。HorticulturalResearchInstitute，ShanghaiAcademyofAgric

5、ulturalSciences；ShanghaiKeyLaboratoryofProtectedHorticulturalTechnology，Shanghai201403，China)Abstract：Tomatocultivar‘1479’，ainbredlinesusceptibletotomatoyellowIeafcurlvirus(TYLCV)，wasusedasatestmaterial，anditsseedlingsweresprayedwith2．0mmol／Lriboflavinbefor

6、einoculatedwithTYLCVtostudytheeffectsofriboflavinontheseedlings’growthandactiveoxygenmetabolism．TheresultsshowedthattheTYLCVinoculationsignificantlyinhibitedtheseedlinggrowth，theseedlingleaves’MDAcontent，O2·一productionrateandH2o2Contentrose，andtheCATandAPXa

7、ctivitiesincreasedwhiletheSODactivitydecreased；ThepretreatmentwithriboflavincouldeffectivelyalleviatetheinhibitingeffectofTYLCVontheseedlinggrowth，significantlydecreasetheseedlings’MDAandH2O2contentsandO2·一productionrateandincreasetheSOD．CATandAPXactivities

8、．Thisindicatedthat2．0mmol／Lriboflavincouldreducetheactiveoxygenproductionoftheseedling1eavesinoculatedwithTYLCV，raisetheactivitiesofantioxidativeenzymes，anddecreasethemembranelipidperoxidation，thusalle