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时间:2017-12-08
《ca(no3)2胁迫对嫁接番茄生长、抗氧化酶活性和活性氧代谢的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、维普资讯http://www.cqvip.com植物营养与肥料学报2008,14(3):527—532P1antNutritionandFertilizerScienceCa(NO3)2胁迫对嫁接番茄生长、抗氧化酶活性和活性氧代谢的影响张古文,朱月林,刘正鲁,魏国平,胡春梅(南京农业大学园艺学院,南京210095)摘要:以“影武者”为砧木,“宝大903”为接穗,在营养液栽培条件下,对80mmol/LCa(NO3)2胁迫下番茄嫁接苗和自根苗的生长、叶片抗氧化酶活性、活性氧代谢以及渗透调节物质含量进行了比较。结果表明,Ca(NO3),胁迫明显抑制植株生长,显著提高植株抗
2、氧化酶活性,显著增加植株0生成速率以及H2Oz、MDA、脯氨酸和可溶性蛋白含量,但胁迫后嫁接苗的生物量显著高于自根苗,抗氧化酶活性、脯氨酸和可溶性蛋白含量均显著高于自根苗,而0生成速率、H2o2和MDA含量则显著低于自根苗。以上结果表明,Ca(NO3)2胁迫下较高的抗氧化酶活性和渗透调节物质含量以及较低的氧化损伤与番茄嫁接苗耐盐性增强有关。关键词:番茄;嫁接;Ca(NO3)2胁迫;膜脂过氧化;营养液栽培中图分类号:$641.2.601文献标识码:A文章编号:1008—505X(2008)03—0527—06EfectofCa(NO3)2stressongrowth,
3、activitiesofantioxidantenzymesandreactiveoxygenmetabolismofgraftedtomatoesZHANGGu-wen,ZHUYue-lin,LIUZheng-lu,WEIGuo-ping,HUChun-mei(DepartmentofHortic~tum,NanjingAgriculturalUniversity,Nanjing210095,China)Abstract:Using“Kagemusya”(LycopersiconesculentumMil1.)asrootstockand“Baoda903”ass
4、cion,graftingwasmadetocomparethedifferencesinplantgrowth,activitiesofantioxidantenzymes,metabolismofreactiveoxygenspeciesandcontentsofosmoticadjustmentsubstancesinleavesbetweengraftedandOWll-—roottomatoseedlingsgrownhydroponi-—callyunder80mmol/LCa(NO3)2stress.TheresultsshowedthatCa(NO3
5、)2stresssignificantlyreducedplantgrowth,butsignificantlyincreasedtheactivitiesofantioxidantenzymes,increasedOproducingrateandcontentsofhydrogenperox-ide(H202),malondiadehyde(MDA),prolineandsolubleproteininbothgraftedandown—rootseedlings.Thebiomassproductionofgraftedseedlings,activities
6、ofantioxidantenzymesandcontentsofprolineandsolubleproteininleavesofgraftedseedlingsweresignificantlyhigherthanthoseofown—rootseedlings,whileOproductionrate,contentsofH202andMDAinleavesofgraftedseedlingsweresignificantlylowerthanthoseofown—rootseedlingsunderCa(NO3)2stress.Theseresultsin
7、dicatedthathigheractivitiesofantioxidantenzymes,highcontentsofosmoticadjustmentsubstancesandlessoxidativedamagemightbeinvolvedinthestrongersalttoleranceofgraftedtomatoseedlings.Keywords:tomato;grafting;Ca(N03)2stress;membranelipidperoxidation;hydroponics近二十年来,我国蔬菜设施栽培发展迅速,然而严重的土壤次生盐渍
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