医用臭氧对骨关节炎患者血清中聚蛋白多糖酶的影响-论文.pdf

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1、中国疼痛医学杂志ChineseJournaIofPainMedicine2013．19(12·727·doi：10．3969／j．issn．1006—9852．2013．12．005医用臭氧对骨关节炎患者血清中聚蛋白多糖酶的影响木刘甬民章沿锋顾鹏程冯智英林向进。祝胜美r浙江大学医学院附属第一医院麻醉科；骨科，杭州310003)摘要目的：探讨臭氧对中晚期骨关节炎(osteoarthritis，OA)患者关节软骨中聚蛋白多糖酶1(aggreca-nase。1，Agase-1orADAMTS-4)及基质金属蛋白酶组织抑制因子(tissueinhibit

2、orofmetalloproteinase3，TIMP．3)的影响。方法：2l例拟接受全膝关节置换术的单关节OA患者为实验组，入院后于手术前接受30％医用臭氧关节腔注射1～2次，间隔3d至术前；另选入院后接受全膝关节置换术的单关节OA而不进行术前臭氧关节腔注射的患者作为空白对照组(18例)。应用酶联免疫吸附试验(enzymelinkedimmunosorbentassay，ELISA)检测Agase1、TIMP一3在两组血清中的表达，以浓度ng／n~表示各项在血清中水平。甲苯胺蓝法检测关节软骨中蛋白多糖(proteoglycan，PG)的含量。结

3、果：与对照组相比，实验组Agase．1表达水平明显下降、TIMP一3明显增加(P<0．05)，但关节软骨中的蛋白多糖含量增加不明显。结论：臭氧可以抑制Agase1的表达、增加TIMP．3的表达，但并不改变OA患者关节软骨中蛋白多糖的含量，未见明显的关节保护作用。关键词：聚蛋白多糖酶；基质金属蛋白酶组织抑制因子；骨关节炎；臭氧LUENCEoIi’oZoNEoNSERUMAGGRECANASEINPAⅡNTSWⅡ’HoSTEoArHRn’ISLIUYong-Min，ZHANGYan-Feng，GUPeng-Cheng2，FENGZhi—Ym'g，LI

4、NXiang-Jin2，ZHUSheng-Mei(DepartmentofAnesthesiology，Departmentoforthopedics，TheFirstAfiliatedHospital，ZhejiangUniversity,Hangzhou310003)Abstract0bjective：Tostudytheinfluenceofozone(03)ontheexpressionofaggrecanase—l(Agase-1)andtissueinhibitorofmetalloproteinase一3(TIMP一3)inseru

5、mofmiddle—latetermosteoarthritis(OA)patients．Methods：Atotalof39casesweredividedintotheozone—treatedgroup(7z=21)inwhichpatientsreceivedtotalkneereplacementsurgerywithozoneinjectionbeforesurgeryandtheblankcontrolgroupr7z=18)inwhichpatientsreceivedtotalkneereplacementsurgerywith

6、outozoneinjection．Ozonewasgivenbykneejointcavityparacentesisonceortwicebeforeoperationafterhospitaladmission．ExpressionofAgase-landTIMP一3inserumofthetwogroupswereinvestigatedbyenzyme—linkedimmunosorbentassay(ELISA)andrepresentedbytheconcentrationunitng／m1．Contentsofproteoglyc

7、an(PG)incartilageweredeterminedbytoluidinebluemethod．Results：Comparedtothecontrolgroup，theserumlevelsofAgase一1intheozone—treatedgroupsignificantlydecreased，andthelevelsofTIMP一3significantlyincreased(P<0．05)，whiletheincreaseinPGincartilagewasnotsignificant．Conclusion：Ozonecans

8、uppressAgase一1andup-regulateTIMP一3incartilageinOApatients，butdidnotc