叶酸偶联的Fe3O4纳米颗粒对活体肿瘤细胞的标记-论文.pdf

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1、Availableonlineatwww．sciencedirect．com，，nansactionsofScienceDirectNonferrousMetaisSocietyofChinaTrans．NonferrousMet．Soc．China23(2013)2079—2084www．tnmsc．cnFolate-conjugatedFe304nanoparticlesforinvivotumorlabelingChun-jiaoZHOU，Shao．huaWANG，YuZHOU，Peng．feiRONG，Zi．

2、ziCHEN，Jin—yanLIU，Jian—daZHOU1．CollegeofScience，HunanAgriculturalUniversity,Changsha410128，China；2．TheThirdXiangyaHospital，CentralSouthUniversity，Changsha410013，ChinaReceived8March2012；accepted10October2012Abstract：HighlybiocompatiblesuperparamagneticFe304nanop

3、articlesweresynthesizedbyamideoffolicacid(FA)ligandsandthel~qn2-groupontothesurfaceofFe304nanoparticles．Theas-synthesizedfolate-conjugatedFe304nanoparticleswerecharacterizedbyX—raydifractiondifractometer,transmissionelectronmicroscope，FT-IRspectrometer,vibratin

4、gsamplemagnetometer,anddynamiclightscatteringinstrument．Theinvivolabelingefectoffolate—conjugatedFe304nanoparticlesonthehepatomacellswasinvestigatedintumor-bearingrat．Theresultsdemonstratethattheas-preparednanoparticleshavecubicstructureOfF0304withapartic1sizeo

5、fabout8nm—andhydrateddiameterof257nmatasaturationmagnetizationof51A·m2／kg．．Thesenanoparticlespossessgoodphysiologicalstability,lowcytotoxicityonhumanskinfibroblastsandnegligibleefectonWistarratsattheconcentrationashighas3mg／kgbodymass．Thefolate-conjugatedFe304n

6、anoparticlescouldbeefectivelymediatedintothehumanhepatomaBel7402cellsthroughthebindingoffolateandfolicacidreceDtor,enhancingthesignalcontrastoftumortissueandsurroundingnormaltissueinMIuimaging．ItisinfavorofthetUlTIOrcellslabeling，tracing，magneticresonanceimagin

7、g(MRI)targetdetectionandmagnetichype~hermia．Keywords：Fe304nanoparticles；superparamagneticproperty；folicacid；tumor；conjugation；magneticresonanceimaging(MIu)；celllabelingco—factorinthesynthesisofseveralenzymesystems．Its1IntroductionmolecularstructureisshowninFig．

8、1．Comparedwithprotein-targetingmoleculessuchasantibodies，folicacidTumorcellmembranesandFe3O4nanoparticlesareisstable，lowcostandnon．immunogenic．Inaddition，bothnegativelycharg