哈茨木霉CGMCC 2979生物转化栀子中的京尼平苷制备京尼平-论文.pdf

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1、ChineseJournalofCatalysis35(2014)1534-1546催化学报2014年第35卷第9期Ivv~tw．chxb．cn毒lI赢le巍藿：．蓉篓叠ed孽。荔m叠薯叠量一一一叠_ll⋯鍪薹。誓曩l羹：junalomg；e；wwweIse、，{e；r．comocae／chnjArticle1■■·■一0‘’■^0。。。‘’●0l0tranStOrmatlOnotgeniposiaeln“raeniaiasminoidestogenlDinbyTrichodermaharzianumCG

2、MCC2979YueshengDong，LepingLiuYongmingBaoapAiyuHaob，YingQin，ZujiaWenajZhilongXiuSchoolofL『厂eScienceandBiotechnology,DalianUniversiofTechnology,Dalian116024Liaoning,ChinaDalianInstituteforDrugCon~olDalian116021，Liaoning,ChinaARTICLEINF0ABSTRACTArticlehistor

3、y：Trichodermaharzianum(harzianum)CGMCC2979wasusedtotransformthegeniposideinGar-Received14February2014deniajasminoidesjasminoides)togenipin，dispensingtheuseofpurifiedenzymeandtheextrac—Accepted4April2014tionofgeniposidefromtherawmateria1．At30。C，pH6．1，andan

4、initialjasminoidesconcentrationPublished20September2014Of80gdriedfruitperliterofmedium．thegeniposide·to-genipinconversionratereached97．8％after48hoffermentation．ThegenipinwaspurifiedfromthefermentationbrothbyacombinedmethodofXAD-16N-resinandsilica-gelchrom

5、atography,eldingatotalrecoveryof62．3％．AKeywords：74．4·kDageniposide-8一glucosidaseimplicatedinthetransformationofgeniposidetogenipinwasGeniposidepurifiedfromharzianumCGMCC2979．Ithadoptimumactivityat50。CandpH4．0—5．0．TheKmGenipinTrichodermaharzianumandVmaxoft

6、heenzymeforgeniposidewere3．6mmol／Land775pmol／h／mgproteinrespectively．BiotransformationThesimple，direct,andeficientbiotransformationofgeniposideinGjasminoidetogenipinbyharzianumCGMCC2979thatisdescribedinthisstudycouldrepresentanalternativeandeffectiveGenip

7、oside—B-glucosidasemethodforproducinggenipin．Purification@2014DalianInstituteofChemicalPhysics，ChineseAcademyofSciences．PublishedbyElsevierB．V．A1lrightsreserved．1．Introductionsuchasgelatin，collagen，andchitosan．Genipinexhibitsacross·linkingpropertycomparab

8、letochemicalcross·-linkingGenipinanditsglycoside，geniposide，constitutethechiefagentssuchasglutaraldehyde，butwithatoxicitythatisaboutactivecomponentsofthefruitsofGardeniajasminoides(Gjas—10000timesless．Genipinhasbeen