吡格列酮对大鼠颅脑损伤后核转录因子、肿瘤坏死因子-α和细胞凋亡的影响-论文.pdf

《吡格列酮对大鼠颅脑损伤后核转录因子、肿瘤坏死因子-α和细胞凋亡的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、·656·ModemPracticalMedicine，June2014，Vo1．26，No．6吡格列酮对大鼠颅脑损伤后核转录因子、肿瘤坏死因子一和细胞凋亡的影响陈慧慧，王永青，彭余江，邵波，朱良才，段红宇，何玺君，李慧勇，杨鹏翔，杨帆【摘要】目的探讨吡格列酮(PGz)干预对大鼠颅脑损伤后脑组织中核转录因子NF．v,B、肿瘤坏死因子一(TNF．a)和细胞凋亡的变化及其可能机制。方法84只健康雄性SD大鼠随机分为PGZ组(仃=42)及对照组=42)，前者通过改良Feeney法建立颅脑损伤模型后立即予以腹腔注射PGZ10mg／kg

2、，对照组则建立模型后予腹腔静脉注射O．9O氯化钠注射液2ml／kg，以后各组分别每24小时腹腔注射一次等量PGZ或0．9％氯化钠注射液，直至动物被处死，根据伤后处死时间随机分为1h、3h、6h、12h、24h、3d和7d共7个亚组，每亚组各6只。取损伤灶周围组织采用免疫组织化学方法检测并比较挫伤灶周围脑组织中NF—rd3及TNF-13．蛋白表达情况，同时采用TUNEL法观察并比较脑挫伤灶周围细胞凋亡情况。结果PGZ组NF—KB及TNf．-ot蛋白表达水平较对照组均明显下降(均P

3、．Ⅱ含量水平为应变量，回归方程Y(PGZ组)=一0．432+0．271X，，=0．947<0．01)；Y(对照组)=一4．168+0．748X，，：0．961(P

4、03InfluenceofpiogUtazoneont~suenucleartranscriptionfactor,tumorneeros~factor-aandcellapoptosisaftertraumaticbraininjuryinratsCHENHuihui,WANGYongqing,PENGYujiang,SHAOBo,ZHULiangcahDUANHongyu,HESijun,LIHu-iyong,YANGPengxiang,YANGFan．(TheFirstPeople'sHospitalofWenling,

5、Taizhou317500,Zhejiang,China)Correspondingauthor：YANGFan,Emaihyangfan5091448@163．com[AbstractlObjectiveToinvestigatethechangesoftissuenucleartranscriptionfactor(NF-Km，tumornecrosisfactor~(TNF-a)andcellapoptosisaftertraumaticbraininjuryandtheinfluenceofpioglitazoneon

6、thesepara-metersinmts．Me-thodsEighty-fourmaleSDratswererandomlydividedintotwogroupsrandomly：pioglitazonegroup(fl=42)andthecontrolgroup=42)．ThepioglitazonegroupwereinducedbyimprovedFeeneymethodandwerereceivedabdominalin-jectionsofpioglitazone(1omg／kg)immediatelyafter

7、injury,andthecontrolgroupwerereceivedabdominalinjectionsofsodiumchlorideinjection(2mVkg)immediatelyaftermjuryandonetimeeverydayuntiltheratsWBSkilled．Eachgroupwasdividedintosevensubgroupsbysacrificedtimeafterinjury,1ll，36}l’12h，24l1'3d，and7dgroup，eachsubgxoupgotsixra

8、ts．Eachsubgroupwererandomlyselectedthreemtsafterbeingkilled,detectedtheexpressionofNF-r．Bandn·oftissuesaroundcontusionbyimmunohistochemica