瑞芬太尼后处理对大鼠心肌缺血再灌注心律失常的影响-论文.pdf

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1、·798·宁夏医学杂志2014年9月第36卷第9期NingxiaMedJ，Sep．2014，Vol36．No．9Doi：10．13621／j．1001—5949．2014．09．0798·论著·瑞芬太尼后处理对大鼠心肌缺血再灌注心律失常的影响陈中青，杨华[摘要]目的观察瑞芬太尼后处理对大鼠心肌缺血再灌注心律失常的影响。方法建立72只大鼠心肌缺血再灌注损伤模型。分为假手术组(Sham组)、缺血再灌注对照组(I—R组)、缺血后处理组(IPO组)和瑞芬太尼不同剂量后处理组(RPO1、RP02、RP03组)，分别以2、4、6Ixg·kg～，静脉泵注5min，停止5min，重复进行3次。记录缺血30r

2、ain、再灌注40rain内心律失常评分，并取右室心肌组织行超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—Px)活力、丙二醛(MDA)及钙离子(Ca“)含量测定。结果与I—R组比较，IPO组、RPOI组、RP02组、RP03组SOD、GSH—Px升高，c含量、MDA降低，心律失常评分降低(P<0．05)；与Sham组比较，I—R组、IPO组、RPO3组SOD、GSH—Px降低，MDA及Ca“含量升高，心律失常评分升高(P<0．05)；IPO组与RP0组比较，各项指标差异无统计学意义(P>0．05)。结论瑞芬太尼可模拟心脏缺血后处理作用，通过抗氧化及抑制钙超载来降低心肌缺血再灌注心律失

3、常的发生。[关键词]瑞芬太尼；缺血后处理；心肌再灌注损伤；心律失常[中图分类号]R614[文献标识码]AEfectsofremifentanilpost—conditioningonarrhythmiasinducedbymyocardialischemiareperfusioninratsCHENZhongqing，rANGHua．1．HeartCenter，GeneralHospitalofNingxiaMedicalUniversity，Yinchuan750004，China；2．Ningx—iaMedicalUniversity，Yinchuan750004，China[Abstr

4、act]ObjectiveTostudytheeffectofremifentanilpost—conditioningonarrhythmiasinducedbymyocardialischemiareperfusioninrats．MethodsThemyocardialischemiareperfusioninjurymodelwasbuiltandtheanimalswererandomlydividedintofourgroups：shamoperationgroup(sham，n=12)；ischemia—reperfusioncontrolgroup(I—R，n=12)；isc

5、hemicpost—conditioninggroup(IPO，n=12)andremifentanilpost—conditioninggroupwhichwasfurtherdividedinto3subgroupsaccordingtothediferentdo—sesofremifentanil(RPOs)．Incontrolgroup，theheartsweresubjectedto30minischemia，followedby90minreperfusion(I／R)．InIPOgrouptheheartsweresubjectedto30minisehemia，followe

6、dbythreeepisodesof5minischemiainsuccessionat5minintervalbe—forereperfusion．InRPOgrouptheheartsweresubjectedto30minischemia，followedby3periodsof5minremifentanilinfusionat2(RPO1，n=12)，4(RPO2，n=12)，6(RPO3，n=12)tzg·kg～at5minintervalbeforereperfusion．Theincidenceofventricularar-rhythmiasandarrhythmiasco

7、reswerealsorecorded．Myocardiumofrightventricleweremadeintotissuehomogenate，thenthesuperoxidedismutase(SOD)andtheactivitiesofglutathioneperoxidase(GSH—Px)andthemal0ndialdehyde(MDA)andthecontentofcalciumion(C