抗体靶向合成型平滑肌细胞 MR探针合成及体外成像的实验研究.pdf

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1、4O卷第lO期Jianl~suMedJ，May1，!Q!·论著抗体靶向合成型平滑肌细胞MR探针合成及体外成像的实验研究陈相汛马占龙尚松安孟欢施海彬【摘要】目的探讨合成型平滑肌细胞单克隆抗体(SMemb)超微超顺磁性MR分子探针作为抗体靶向特异性探针体外MR成像的可行性。方法采用EDC／NHS化学偶联方法将SMemb与USPIO颗粒相结合，并测定其电子粒径、水合粒径、Zeta电位及弛豫效能等表征特性。SMemb标记探针(SMemb-USPIO)为实验组，未标记USPIO探针(USPIO)为对照组，PBS溶液为空白组。实验组及对照组探针分别与不同铁浓度探针恒温孵育，普鲁士蓝染色鉴定细胞内

2、铁，确定最佳标记浓度。实验组和对照组探针与合成型VSMC共孵育，在7．OTMRI行体外MRIT2WI成像。结果抗体靶向探针与USPIO颗粒磁性相似，稳定性高。实验组及对照组各浓度间蓝染率有统计学差异(P

3、0—1130—04Aexperimentalstudyofproliferativesmoothmusclecellmonoclonalantibody-targetedprobeforinvitroMRimagingCHENXiangxun，MAZhanlong，SHANGSongan，eta1．DepartmentofRadiology，FtrstAfiliatedHospital，NaniingMedicalUniversiy，Nanjing210029，CHINA[Abstract]ObjectiveToinvestigatethefeasibilityofprolifera

4、tivesmoothmusclecellmonoclonalantibody-targetedprobeforinvitroMRimaging．MethodsTheproliferativesmoothmusclecellmonoclonalantibody(SMemb)wascombinedwithDMSAcoatedUSPIOthroughEDC／NHSchemicalcouplingmethodsandtheelectronicparticle，hydratedparticlesize，zetapotential，andrelaxivityvalueweremeasured．T

5、heSMemb-eonjugatedprobe(SMemirUSPIO)wasdesignedasexperimenta1group(A)，uneougatedprobe(USPIO)asthecontro1(groupC)andPBSsolutionasblankcontrolgroup(BC)．TheVSMCwasincubatedwithSMemirUSPIOindifferentconcentrations，andprussianbluestainingwasperformedtoshowtheintracellulariron(Fe)andfindtheoptimal1ab

6、elingconcentratim~DifferentconcentrationsofPMSCculturedwithgroupsofAandCwerescreenedusing7．OTMRonT2WIinvitro．TheintraeellularironlabelingefficiencyamongdifferentironconcentrationsandT2signalwerecompared．ResultsThesyntheticVSMCwassuccessfullyisolatedandculturedinvitro，whichhighlyexpressedSMembpr

7、otein．SimilarmagnetismandstabilitywerefoundbothintheSMemb—USPIOandUSPIO(P>0．05)．ThebluestainingratesamongdifferentconcentratinsofFeweredifferentamongdifferentFeconcentrationsingroupsofAandC(P<0．05)．TheT2WIimagesofgroupAwerelowerth