换锦花种胚和叶片的组织培养研究

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1、江西农业大学学报2011，33(4):0665－0669http://xuebao．jxau．edu．cnActaAgriculturaeUniversitatisJiangxiensisE－mail:ndxb7775@sina．com换锦花种胚和叶片的组织培养研究11*2111时剑，童再康，刘志高，高燕会，姜小凤，黄华宏(1．浙江农林大学亚热带森林培育国家重点实验室，浙江临安311300;2．浙江农林大学园林学院，浙江临安311300)摘要:换锦花是我国南方的优良球根花卉和重要的药用植物。采用组织培养技术以换锦花种胚和叶片为外植体，对其进行研究，结果显示:换锦花种胚的诱导率在各培养基

2、上均较高，平均为75．8%，适宜的愈伤组织诱导培养基为MS+6－BA2mg/L+2，4－D2mg/L+蔗糖60g/L，适宜的丛芽诱导培养基为MS+6－BA2mg/L+2，4－D1mg/L+蔗糖30g/L;换锦花成熟叶段愈伤组织的平均诱导率为13．5%，而幼嫩叶段的平均诱导率为65．8%，两者差异极显著，适宜于幼嫩叶段诱导的培养基为MS+6－BA20mg/L+NAA1mg/L+蔗糖30g/L，各生长调节剂配比的试验结果显示，NAA较高时利于根的诱导，而6－BA较高时利于芽的诱导。关键词:换锦花;石蒜属;组织培养;种胚;叶片+中图分类号:Q949．718．25;Q943．1文献标志码:A文

3、章编号:1000－2286(2011)04－0665－05TissueCultureofLeavesandEmbryoinLycorissprengeri11*2SHIJian，TONGZai-kang，LIUZhi-gao，111GAOYan-hui，JIANGXiao-feng，HUANGHua-hong(1．ZhejiangAgricultureandForestryUniversity，theNurturingStationfortheStateKeyLaboratoryofSubtropicalSilviculture，Linan311300，China;2．Zhejiang

4、AgricultureandForestryUniversity，LandscapeAr-chitectureCollege，Linan311300，China)Abstract:LycorissprengeriComesexbakerisoneofthemostbeautifulbulbsbuthasn’tbeenusedinlandscapingwidelyowingtolackofrapidpropagationtechnique．TheleafbladesandembryoofL．sprengeriwereculturedinvitroonMSmediumssupplement

5、edwithdifferentconcentrationsofplantgrowthregulator．Theresultsshowedthathighinductionrates，75．8%onaverage，wereobtainedinembryoculture．ThemostidealcallusinductionmediumforembryoculturewasMS+6－BA2mg/L+2，4－D2mg/L+Sucrose60g/L，whilethemostidealclusteredshootsinductionmediumwasMS+6－BA2mg/L+2，4－D1mg/L

6、+Su-crose30g/L．Theresultsofleaftissuecultureshowedthattheinductionrateofyoungleaveswas65．8%onaveragewhichwashigherthanthatofmatureleaves(13．5%onaverage)．ThemostidealinductionmediumforyoungleavestissueculturewasMS+6－BA20mg/L+NAA1mg/L+Sucrose30g/L．Theresultsofgrowthregulatorcontrasttestshowedthath

7、igherNAAconcentrationwasgoodforinductionofroots，whilehigher6－BAconcentrationwasgoodforinductionofshoots．Keywords:L．sprengeri;LycorisHerb．;tissueculture;embryo;leaves收稿日期:2011－02－10修回日期:2011－05－08基金项目:浙江省科技项目(2006C12003)、浙江省创